Title |
Strain engineering for improved expression of recombinant proteins in bacteria
|
---|---|
Published in |
Microbial Cell Factories, May 2011
|
DOI | 10.1186/1475-2859-10-32 |
Pubmed ID | |
Authors |
Tomohiro Makino, Georgios Skretas, George Georgiou |
Abstract |
Protein expression in Escherichia coli represents the most facile approach for the preparation of non-glycosylated proteins for analytical and preparative purposes. So far, the optimization of recombinant expression has largely remained a matter of trial and error and has relied upon varying parameters, such as expression vector, media composition, growth temperature and chaperone co-expression. Recently several new approaches for the genome-scale engineering of E. coli to enhance recombinant protein expression have been developed. These methodologies now enable the generation of optimized E. coli expression strains in a manner analogous to metabolic engineering for the synthesis of low-molecular-weight compounds. In this review, we provide an overview of strain engineering approaches useful for enhancing the expression of hard-to-produce proteins, including heterologous membrane proteins. |
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Unknown | 1 | 100% |
Demographic breakdown
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
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United Kingdom | 3 | <1% |
Canada | 2 | <1% |
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Germany | 2 | <1% |
India | 1 | <1% |
South Africa | 1 | <1% |
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Other | 3 | <1% |
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Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 123 | 24% |
Researcher | 95 | 18% |
Student > Master | 64 | 12% |
Student > Bachelor | 56 | 11% |
Other | 21 | 4% |
Other | 68 | 13% |
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Chemical Engineering | 9 | 2% |
Other | 33 | 6% |
Unknown | 98 | 19% |