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A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector

Overview of attention for article published in BMC Molecular and Cell Biology, August 2013
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  • Good Attention Score compared to outputs of the same age and source (66th percentile)

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4 X users

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86 Mendeley
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1 CiteULike
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Title
A plasmid toolkit for cloning chimeric cDNAs encoding customized fusion proteins into any Gateway destination expression vector
Published in
BMC Molecular and Cell Biology, August 2013
DOI 10.1186/1471-2199-14-18
Pubmed ID
Authors

Raquel Buj, Noa Iglesias, Anna M Planas, Tomàs Santalucía

Abstract

Valuable clone collections encoding the complete ORFeomes for some model organisms have been constructed following the completion of their genome sequencing projects. These libraries are based on Gateway cloning technology, which facilitates the study of protein function by simplifying the subcloning of open reading frames (ORF) into any suitable destination vector. The expression of proteins of interest as fusions with functional modules is a frequent approach in their initial functional characterization. A limited number of Gateway destination expression vectors allow the construction of fusion proteins from ORFeome-derived sequences, but they are restricted to the possibilities offered by their inbuilt functional modules and their pre-defined model organism-specificity. Thus, the availability of cloning systems that overcome these limitations would be highly advantageous.

X Demographics

X Demographics

The data shown below were collected from the profiles of 4 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 86 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 1%
France 1 1%
Brazil 1 1%
Slovenia 1 1%
China 1 1%
United States 1 1%
Unknown 80 93%

Demographic breakdown

Readers by professional status Count As %
Researcher 22 26%
Student > Ph. D. Student 18 21%
Student > Master 7 8%
Student > Doctoral Student 7 8%
Student > Bachelor 5 6%
Other 18 21%
Unknown 9 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 39 45%
Biochemistry, Genetics and Molecular Biology 25 29%
Medicine and Dentistry 6 7%
Unspecified 2 2%
Pharmacology, Toxicology and Pharmaceutical Science 1 1%
Other 4 5%
Unknown 9 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 28 August 2013.
All research outputs
#14,474,215
of 25,374,917 outputs
Outputs from BMC Molecular and Cell Biology
#641
of 1,233 outputs
Outputs of similar age
#108,821
of 210,086 outputs
Outputs of similar age from BMC Molecular and Cell Biology
#4
of 12 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 42nd percentile – i.e., 42% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,233 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 47th percentile – i.e., 47% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 210,086 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 66% of its contemporaries.