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Evaluating Extracellular Matrix influence on adherent cell signaling by Cold Trypsin Phosphorylation-specific Flow Cytometry

Overview of attention for article published in BMC Molecular and Cell Biology, August 2013
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Title
Evaluating Extracellular Matrix influence on adherent cell signaling by Cold Trypsin Phosphorylation-specific Flow Cytometry
Published in
BMC Molecular and Cell Biology, August 2013
DOI 10.1186/1471-2121-14-36
Pubmed ID
Authors

Iren Abrahamsen, James B Lorens

Abstract

Tissue microenvironments comprise different extracellular matrix (ECM) proteins that regulate cellular responsiveness to growth factors. In vitro culture of adherent cells on ECM-coated substrata is commonly used to study microenvironmental influence on specific cell signaling responses. Phosphorylation-specific flow cytometry can be utilized to quantify intracellular phosphorylation-dependent signaling events in single cells. However this approach necessitates trypsinization of adherent cells to accommodate flow cytometric analysis. Trypsin is a potent activator of cell signaling and can obscure signal transduction events induced by other factors.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 31 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United Kingdom 1 3%
Japan 1 3%
Colombia 1 3%
Unknown 28 90%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 11 35%
Researcher 6 19%
Professor > Associate Professor 4 13%
Other 3 10%
Student > Master 1 3%
Other 2 6%
Unknown 4 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 39%
Engineering 4 13%
Medicine and Dentistry 3 10%
Chemistry 2 6%
Biochemistry, Genetics and Molecular Biology 2 6%
Other 3 10%
Unknown 5 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 August 2013.
All research outputs
#17,286,645
of 25,374,917 outputs
Outputs from BMC Molecular and Cell Biology
#778
of 1,233 outputs
Outputs of similar age
#132,139
of 209,783 outputs
Outputs of similar age from BMC Molecular and Cell Biology
#6
of 12 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one is in the 21st percentile – i.e., 21% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,233 research outputs from this source. They receive a mean Attention Score of 4.0. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 209,783 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 27th percentile – i.e., 27% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 12 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.