Title |
Fine mapping of V(D)J recombinase mediated rearrangements in human lymphoid malignancies
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Published in |
BMC Genomics, August 2013
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DOI | 10.1186/1471-2164-14-565 |
Pubmed ID | |
Authors |
Eitan Halper-Stromberg, Jared Steranka, Nicolas Giraldo-Castillo, Timothy Fuller, Stephen Desiderio, Kathleen H Burns |
Abstract |
Lymphocytes achieve diversity in antigen recognition in part by rearranging genomic DNA at loci encoding antibodies and cell surface receptors. The process, termed V(D)J recombination, juxtaposes modular coding sequences for antigen binding. Erroneous recombination events causing chromosomal translocations are recognized causes of lymphoid malignancies. Here we show a hybridization based method for sequence enrichment can be used to efficiently and selectively capture genomic DNA adjacent to V(D)J recombination breakpoints for massively parallel sequencing. The approach obviates the need for PCR amplification of recombined sequences. |
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France | 1 | 50% |
Demographic breakdown
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Scientists | 2 | 100% |
Mendeley readers
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Unknown | 29 | 94% |
Demographic breakdown
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Student > Ph. D. Student | 11 | 35% |
Researcher | 7 | 23% |
Student > Master | 4 | 13% |
Student > Doctoral Student | 2 | 6% |
Professor | 2 | 6% |
Other | 4 | 13% |
Unknown | 1 | 3% |
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Biochemistry, Genetics and Molecular Biology | 8 | 26% |
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Neuroscience | 1 | 3% |
Other | 1 | 3% |
Unknown | 2 | 6% |