Title |
Use of a promiscuous, constitutively-active bacterial enhancer-binding protein to define the σ54 (RpoN) regulon of Salmonella Typhimurium LT2
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Published in |
BMC Genomics, September 2013
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DOI | 10.1186/1471-2164-14-602 |
Pubmed ID | |
Authors |
David J Samuels, Jonathan G Frye, Steffen Porwollik, Michael McClelland, Jan Mrázek, Timothy R Hoover, Anna C Karls |
Abstract |
Sigma54, or RpoN, is an alternative σ factor found widely in eubacteria. A significant complication in analysis of the global σ⁵⁴ regulon in a bacterium is that the σ⁵⁴ RNA polymerase holoenzyme requires interaction with an active bacterial enhancer-binding protein (bEBP) to initiate transcription at a σ⁵⁴-dependent promoter. Many bacteria possess multiple bEBPs, which are activated by diverse environmental stimuli. In this work, we assess the ability of a promiscuous, constitutively-active bEBP-the AAA+ ATPase domain of DctD from Sinorhizobium meliloti-to activate transcription from all σ⁵⁴-dependent promoters for the characterization of the σ⁵⁴ regulon of Salmonella Typhimurium LT2. |
X Demographics
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France | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
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Unknown | 53 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 13 | 25% |
Researcher | 9 | 17% |
Student > Master | 6 | 11% |
Professor | 5 | 9% |
Student > Bachelor | 3 | 6% |
Other | 8 | 15% |
Unknown | 9 | 17% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 21 | 40% |
Biochemistry, Genetics and Molecular Biology | 19 | 36% |
Immunology and Microbiology | 3 | 6% |
Unspecified | 1 | 2% |
Unknown | 9 | 17% |