Title |
Sensitivity of a real-time PCR method for the detection of transgenes in a mixture of transgenic and non-transgenic seeds of papaya (Carica papayaL.)
|
---|---|
Published in |
BMC Biotechnology, September 2013
|
DOI | 10.1186/1472-6750-13-69 |
Pubmed ID | |
Authors |
Madhugiri Nageswara-Rao, Charles Kwit, Sujata Agarwal, Mariah T Patton, Jordan A Skeen, Joshua S Yuan, Richard M Manshardt, C Neal Stewart |
Abstract |
Genetically engineered (GE) ringspot virus-resistant papaya cultivars 'Rainbow' and 'SunUp' have been grown in Hawai'i for over 10 years. In Hawai'i, the introduction of GE papayas into regions where non-GE cultivars are grown and where feral non-GE papayas exist have been accompanied with concerns associated with transgene flow. Of particular concern is the possibility of transgenic seeds being found in non-GE papaya fruits via cross-pollination. Development of high-throughput methods to reliably detect the adventitious presence of such transgenic material would benefit both the scientific and regulatory communities. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Sweden | 1 | 2% |
Bangladesh | 1 | 2% |
South Africa | 1 | 2% |
Unknown | 47 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 10 | 20% |
Student > Bachelor | 9 | 18% |
Student > Doctoral Student | 7 | 14% |
Student > Ph. D. Student | 4 | 8% |
Student > Master | 4 | 8% |
Other | 9 | 18% |
Unknown | 7 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 28 | 56% |
Biochemistry, Genetics and Molecular Biology | 5 | 10% |
Engineering | 4 | 8% |
Environmental Science | 1 | 2% |
Veterinary Science and Veterinary Medicine | 1 | 2% |
Other | 2 | 4% |
Unknown | 9 | 18% |