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X Demographics
Mendeley readers
Attention Score in Context
Title |
Bovine serum albumin further enhances the effects of organic solvents on increased yield of polymerase chain reaction of GC-rich templates
|
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Published in |
BMC Research Notes, May 2012
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DOI | 10.1186/1756-0500-5-257 |
Pubmed ID | |
Authors |
Eric M Farell, Gladys Alexandre |
Abstract |
While being a standard powerful molecular biology technique, applications of the PCR to the amplification of high GC-rich DNA samples still present challenges which include limited yield and poor specificity of the reaction. Organic solvents, including DMSO and formamide, have been often employed as additives to increase the efficiency of amplification of high GC content (GC > 60%) DNA sequences. Bovine serum albumin (BSA) has been used as an additive in several applications, including restriction enzyme digestions as well as in PCR amplification of templates from environmental samples that contain potential inhibitors such as phenolic compounds. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 67% |
Unknown | 1 | 33% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 2 | 67% |
Members of the public | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 482 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 4 | <1% |
Brazil | 2 | <1% |
Austria | 2 | <1% |
Germany | 2 | <1% |
United Kingdom | 2 | <1% |
Malaysia | 1 | <1% |
Canada | 1 | <1% |
New Zealand | 1 | <1% |
Slovenia | 1 | <1% |
Other | 5 | 1% |
Unknown | 461 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 91 | 19% |
Student > Bachelor | 87 | 18% |
Student > Master | 77 | 16% |
Researcher | 60 | 12% |
Student > Doctoral Student | 29 | 6% |
Other | 53 | 11% |
Unknown | 85 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 179 | 37% |
Biochemistry, Genetics and Molecular Biology | 118 | 24% |
Environmental Science | 26 | 5% |
Immunology and Microbiology | 12 | 2% |
Chemistry | 9 | 2% |
Other | 42 | 9% |
Unknown | 96 | 20% |
Attention Score in Context
This research output has an Altmetric Attention Score of 24. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 October 2022.
All research outputs
#1,540,685
of 25,116,143 outputs
Outputs from BMC Research Notes
#176
of 4,494 outputs
Outputs of similar age
#8,630
of 170,129 outputs
Outputs of similar age from BMC Research Notes
#6
of 80 outputs
Altmetric has tracked 25,116,143 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,494 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.1. This one has done particularly well, scoring higher than 96% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 170,129 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 94% of its contemporaries.
We're also able to compare this research output to 80 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 93% of its contemporaries.