Title |
MicroRNA-21 promotes proliferation, migration, and invasion of colorectal cancer, and tumor growth associated with down-regulation of sec23a expression
|
---|---|
Published in |
BMC Cancer, August 2016
|
DOI | 10.1186/s12885-016-2628-z |
Pubmed ID | |
Authors |
Chenli Li, Lingxu Zhao, Yuan Chen, Tiantian He, Xiaowan Chen, Jiating Mao, Chunmei Li, Jianxin Lyu, Qing H. Meng |
Abstract |
MicroRNA-21 (miR-21) is up-regulated in many cancers, including colorectal cancer (CRC). Nevertheless, the function of miR-21 in CRC and the mechanism underlying that function is still unclear. After analyzing the expression of miR-21 and Sec23A in CRC cell lines, we transfected the highest miR-21 expressing cell line, SW-480, with a plasmid containing an miR-21 inhibitor and the lowest miR-21 expressing cell line, DLD-1, with a plasmid containing an miR-21 mimic and measured the effects on the expression of Sec23A and on cell proliferation, migration, and invasion. We also evaluated the effect of knocking down Sec23A on miR-21 expression and its effects on cell proliferation, migration, and invasion. Finally, we assessed the effect of miR-21 in a xenograft tumor model in mice. Tumor tissues from these mice were subjected to immunohistochemical staining to detect the expression of Sec23A. Genetic deletion of miR-21 suppressed the proliferation, migration, and invasion of SW-480 cells, while over-expression of miR-21 promoted proliferation, migration, and invasion of DLD-1 cells. Inhibition of miR-21 increased the expression of Sec23A protein in SW-480 cells while over-expression of miR-21 significantly suppressed the expression of Sec23A protein and Sec23A mRNA in DLD-1 cells. Knockdown of Sec23A increased the expression of miR-21 in SW480 and DLD-1 cells and their proliferation (DLD-1 only), migration, and invasion. Over-expression of miR-21 promoted tumor growth in BALB/c nude mice and suppressed tumor expression of Sec23A. These findings provide novel insight into the molecular functions of miR-21 in CRC, which may serve as a potential interesting target. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 37 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 8 | 22% |
Student > Ph. D. Student | 8 | 22% |
Student > Bachelor | 4 | 11% |
Student > Doctoral Student | 2 | 5% |
Student > Postgraduate | 2 | 5% |
Other | 4 | 11% |
Unknown | 9 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 24% |
Agricultural and Biological Sciences | 7 | 19% |
Medicine and Dentistry | 5 | 14% |
Nursing and Health Professions | 2 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 3% |
Other | 3 | 8% |
Unknown | 10 | 27% |