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Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS…

Overview of attention for article published in Microbial Cell Factories, September 2013
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Title
Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF
Published in
Microbial Cell Factories, September 2013
DOI 10.1186/1475-2859-12-86
Pubmed ID
Authors

Alberto Rodriguez, Juan A Martínez, José L Báez-Viveros, Noemí Flores, Georgina Hernández-Chávez, Octavio T Ramírez, Guillermo Gosset, Francisco Bolivar

Abstract

During the last two decades many efforts have been directed towards obtaining efficient microbial processes for the production of shikimic acid (SA); however, feeding high amounts of substrate to increase the titer of this compound has invariably rendered low conversion yields, leaving room for improvement of the producing strains. In this work we report an alternative platform to overproduce SA in a laboratory-evolved Escherichia coli strain, based on plasmid-driven constitutive expression of six genes selected from the pentose phosphate and aromatic amino acid pathways, artificially arranged as an operon. Production strains also carried inactivated genes coding for phosphotransferase system components (ptsHIcrr), shikimate kinases I and II (aroK and aroL), pyruvate kinase I (pykF) and the lactose operon repressor (lacI).

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X Demographics

The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Indonesia 1 1%
Mexico 1 1%
China 1 1%
Unknown 68 96%

Demographic breakdown

Readers by professional status Count As %
Student > Master 17 24%
Student > Ph. D. Student 11 15%
Researcher 7 10%
Student > Bachelor 6 8%
Student > Doctoral Student 2 3%
Other 7 10%
Unknown 21 30%
Readers by discipline Count As %
Agricultural and Biological Sciences 22 31%
Biochemistry, Genetics and Molecular Biology 15 21%
Engineering 2 3%
Chemistry 2 3%
Social Sciences 2 3%
Other 4 6%
Unknown 24 34%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 October 2013.
All research outputs
#15,280,625
of 22,723,682 outputs
Outputs from Microbial Cell Factories
#978
of 1,588 outputs
Outputs of similar age
#126,811
of 205,843 outputs
Outputs of similar age from Microbial Cell Factories
#7
of 9 outputs
Altmetric has tracked 22,723,682 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,588 research outputs from this source. They receive a mean Attention Score of 4.4. This one is in the 30th percentile – i.e., 30% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 205,843 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 9 others from the same source and published within six weeks on either side of this one. This one has scored higher than 2 of them.