Title |
Constitutive expression of selected genes from the pentose phosphate and aromatic pathways increases the shikimic acid yield in high-glucose batch cultures of an Escherichia coli strain lacking PTS and pykF
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Published in |
Microbial Cell Factories, September 2013
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DOI | 10.1186/1475-2859-12-86 |
Pubmed ID | |
Authors |
Alberto Rodriguez, Juan A Martínez, José L Báez-Viveros, Noemí Flores, Georgina Hernández-Chávez, Octavio T Ramírez, Guillermo Gosset, Francisco Bolivar |
Abstract |
During the last two decades many efforts have been directed towards obtaining efficient microbial processes for the production of shikimic acid (SA); however, feeding high amounts of substrate to increase the titer of this compound has invariably rendered low conversion yields, leaving room for improvement of the producing strains. In this work we report an alternative platform to overproduce SA in a laboratory-evolved Escherichia coli strain, based on plasmid-driven constitutive expression of six genes selected from the pentose phosphate and aromatic amino acid pathways, artificially arranged as an operon. Production strains also carried inactivated genes coding for phosphotransferase system components (ptsHIcrr), shikimate kinases I and II (aroK and aroL), pyruvate kinase I (pykF) and the lactose operon repressor (lacI). |
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