Title |
Preliminary results in quantitation of HLA-DRA by real-time PCR: a promising approach to identify immunosuppression in sepsis
|
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Published in |
Critical Care, October 2013
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DOI | 10.1186/cc13046 |
Pubmed ID | |
Authors |
Sara Cajander, Anders Bäckman, Elisabet Tina, Kristoffer Strålin, Bo Söderquist, Jan Källman |
Abstract |
Reduced monocyte human leukocyte antigen (mHLA)-DR surface expression in the late phase of sepsis is postulated as a general biomarker of sepsis-induced immunosuppression and an independent predictor of nosocomial infections.However, traditional monitoring of mHLA-DR by flow cytometry has disadvantages due to specific laboratory requirements. An mRNA-based HLA-DR monitoring by polymerase chain reaction (PCR) would improve the clinical usage and facilitate conduction of large multicenter studies. In this study, we evaluated an mRNA-based HLA-DR monitoring by quantitative real-time PCR (qRT-PCR) as an alternative method to traditional flow cytometry. |
Mendeley readers
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Demographic breakdown
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