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X Demographics
Mendeley readers
Attention Score in Context
| Title |
A novel real-time PCR assay for specific detection of Brucella melitensis
|
|---|---|
| Published in |
BMC Infectious Diseases, March 2017
|
| DOI | 10.1186/s12879-017-2327-7 |
| Pubmed ID | |
| Authors |
Rene Kaden, Sevinc Ferrari, Erik Alm, Tara Wahab |
| Abstract |
Brucellosis is a zoonosis that occurs worldwide. The disease has been completely eradicated in livestock in Sweden in 1994, and all cases of confirmed human brucellosis are imported into Sweden from other countries. However, due to an increase in the number of refugees and asylum seekers from the middle-east to Sweden, there is a need to improve the current diagnostic methodology for Brucella melitensis. Whilst culture of Brucella species can be used as a diagnostic tool, real-time PCR approaches provide a much faster result. The aim of this study was to set up a species-specific real-time PCR for the detection of all biovars of Brucella melitensis, which could be used routinely in diagnostic laboratories. A Brucella melitensis real-time PCR assay was designed using all available genomes in the public database of Brucella (N = 96) including all complete genomes of Brucella melitensis (N = 17). The assay was validated with a collection of 37 Brucella species reference strains, 120 Brucella melitensis human clinical isolates, and 45 clinically relevant non-Brucella melitensis strains. In this study we developed a single real-time PCR for the specific detection of all biovars of Brucella melitensis. This new real-time PCR method shows a high specificity (100%) and a high sensitivity (1.25 GE/μl) and has been implemented in the laboratories of four governmental authorities across Sweden. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 2 | 67% |
| Venezuela, Bolivarian Republic of | 1 | 33% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 67% |
| Scientists | 1 | 33% |
Mendeley readers
The data shown below were compiled from readership statistics for 68 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Malaysia | 1 | 1% |
| Unknown | 67 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 11 | 16% |
| Student > Ph. D. Student | 10 | 15% |
| Student > Master | 8 | 12% |
| Student > Bachelor | 8 | 12% |
| Student > Doctoral Student | 3 | 4% |
| Other | 12 | 18% |
| Unknown | 16 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 10 | 15% |
| Veterinary Science and Veterinary Medicine | 9 | 13% |
| Immunology and Microbiology | 9 | 13% |
| Medicine and Dentistry | 8 | 12% |
| Biochemistry, Genetics and Molecular Biology | 7 | 10% |
| Other | 7 | 10% |
| Unknown | 18 | 26% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 August 2017.
All research outputs
#14,928,316
of 22,961,203 outputs
Outputs from BMC Infectious Diseases
#4,125
of 7,707 outputs
Outputs of similar age
#184,627
of 309,205 outputs
Outputs of similar age from BMC Infectious Diseases
#104
of 168 outputs
Altmetric has tracked 22,961,203 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 7,707 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 9.6. This one is in the 41st percentile – i.e., 41% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 309,205 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 37th percentile – i.e., 37% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 168 others from the same source and published within six weeks on either side of this one. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.