Title |
MiRNA expression in the cartilage of patients with osteoarthritis
|
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Published in |
Journal of Orthopaedic Surgery and Research, March 2017
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DOI | 10.1186/s13018-017-0542-y |
Pubmed ID | |
Authors |
Marta Kopańska, Dariusz Szala, Joanna Czech, Natalia Gabło, Krzysztof Gargasz, Mateusz Trzeciak, Izabela Zawlik, Sławomir Snela |
Abstract |
Osteoarthritis (OA), the most prevalent disease of articulating joints, is a complex multifactorial disease caused by genetic, mechanical, and environmental factors. In this research, we evaluated miRNA expression in OA. Forty tissue samples from 29 patients undergoing joint replacement for OA were evaluated. Tissue from two control patients undergoing hip replacement not related to OA was used as a control. Total RNA (containing miRNA species) from cartilage was isolated using a mirVana miRNA Isolation Kit. Expression of 19 miRNAs was assessed by real-time quantitative polymerase chain reaction. Expression of four miRNAs, miR-138-5p, miR-146a-5p, miR-335-5p, and miR-9-5p, was significantly upregulated in OA tissues (patients vs. control group). These findings may contribute to disease prevention and the development of therapeutic targets for OA. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 41 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 15% |
Student > Bachelor | 6 | 15% |
Researcher | 5 | 12% |
Student > Postgraduate | 4 | 10% |
Other | 3 | 7% |
Other | 6 | 15% |
Unknown | 11 | 27% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 9 | 22% |
Medicine and Dentistry | 6 | 15% |
Agricultural and Biological Sciences | 4 | 10% |
Nursing and Health Professions | 2 | 5% |
Immunology and Microbiology | 2 | 5% |
Other | 6 | 15% |
Unknown | 12 | 29% |