Title |
Immunodetection of cells with a CD44+/CD24- phenotype in canine mammary neoplasms
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Published in |
BMC Veterinary Research, October 2013
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DOI | 10.1186/1746-6148-9-205 |
Pubmed ID | |
Authors |
GeórgiaModé Magalhães, ErikaMaria Terra, Rosemeri de Oliveira Vasconcelos, Márcio de Barros Bandarra, PamelaRodriguesReina Moreira, Mayara Caroline Rosolem, Antonio Carlos Alessi |
Abstract |
Cancer stem cells (CSCs) are able to self-renew and to form metastases. Using flow cytometry, CSCs were detected in canine mammary tumors as cells CD44(+) and CD24(-). The aim of this study was to detect these CSCs by immunohistochemistry and correlate their frequency with canine mammary neoplasm grade and histopathological type.130 mammary neoplasm samples were selected from tissue blocks at the Department of Pathology at UNESP and classified according to (BJVP 4:153-180, 2011). These samples were composed by adenomas, lymph node metastases, solid carcinomas grades II and III, tubular, papillary and carcinomas in mixed tumor grades I, II and III. Immunohistochemistry was performed with antibodies against CD44 and CD24. Linear regression was performed using Pearson's correlation test. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 40% |
Venezuela, Bolivarian Republic of | 2 | 40% |
Unknown | 1 | 20% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 5 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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United States | 1 | 2% |
Italy | 1 | 2% |
Unknown | 46 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Postgraduate | 10 | 21% |
Other | 9 | 19% |
Researcher | 5 | 10% |
Student > Master | 5 | 10% |
Student > Ph. D. Student | 4 | 8% |
Other | 11 | 23% |
Unknown | 4 | 8% |
Readers by discipline | Count | As % |
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Veterinary Science and Veterinary Medicine | 23 | 48% |
Medicine and Dentistry | 10 | 21% |
Agricultural and Biological Sciences | 4 | 8% |
Immunology and Microbiology | 2 | 4% |
Chemical Engineering | 1 | 2% |
Other | 2 | 4% |
Unknown | 6 | 13% |