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Mendeley readers
Attention Score in Context
| Title |
The establishment of a duplex real-time PCR assay for rapid and simultaneous detection of blaNDM and blaKPC genes in bacteria
|
|---|---|
| Published in |
Annals of Clinical Microbiology and Antimicrobials, October 2013
|
| DOI | 10.1186/1476-0711-12-30 |
| Pubmed ID | |
| Authors |
Fen Zheng, Jingjing Sun, Cancan Cheng, Yongyu Rui |
| Abstract |
The latest threat of multidrug-resistant Gram-negative bacteria corresponds to the emergence of carbapenemase New Delhi metallo-β-lactamase (NDM) and Klebsiella pneumoniae carbapenemase (KPC) producers. Rapid molecular detection is essential to limit their spread. In this study, a duplex real-time polymerase chain reaction (PCR) that was specific for the detection of blaNDM and blaKPC with the same limit of detection of ten plasmid copies was developed. The assay was linear over eight log dilutions for blaNDM (R2 = 0.971; slope, -3.273) and blaKPC (R2 = 0.992; slope, -2.997) with efficiencies of 102% and 115%, respectively. The assay was validated with 157 clinical isolates and showed 100% concordance with conventional PCR. The excellent performance of the duplex PCR assay makes it a powerful tool for surveillance of the carbapenemases NDM and KPC. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 27 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 27 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Doctoral Student | 5 | 19% |
| Researcher | 4 | 15% |
| Student > Master | 4 | 15% |
| Student > Ph. D. Student | 3 | 11% |
| Student > Bachelor | 3 | 11% |
| Other | 2 | 7% |
| Unknown | 6 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 7 | 26% |
| Biochemistry, Genetics and Molecular Biology | 4 | 15% |
| Medicine and Dentistry | 4 | 15% |
| Immunology and Microbiology | 3 | 11% |
| Engineering | 1 | 4% |
| Other | 0 | 0% |
| Unknown | 8 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 October 2013.
All research outputs
#18,351,676
of 22,727,570 outputs
Outputs from Annals of Clinical Microbiology and Antimicrobials
#457
of 606 outputs
Outputs of similar age
#157,975
of 212,053 outputs
Outputs of similar age from Annals of Clinical Microbiology and Antimicrobials
#4
of 8 outputs
Altmetric has tracked 22,727,570 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 606 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.3. This one is in the 12th percentile – i.e., 12% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 212,053 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 8 others from the same source and published within six weeks on either side of this one. This one has scored higher than 4 of them.