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Microglia activation is essential for BMP7-mediated retinal reactive gliosis

Overview of attention for article published in Journal of Neuroinflammation, April 2017
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Title
Microglia activation is essential for BMP7-mediated retinal reactive gliosis
Published in
Journal of Neuroinflammation, April 2017
DOI 10.1186/s12974-017-0855-0
Pubmed ID
Authors

Subramanian Dharmarajan, Debra L. Fisk, Christine M. Sorenson, Nader Sheibani, Teri L. Belecky-Adams

Abstract

Our previous studies have shown that BMP7 is able to trigger activation of retinal macroglia. However, these studies showed the responsiveness of Müller glial cells and retinal astrocytes in vitro was attenuated in comparison to those in vivo, indicating other retinal cell types may be mediating the response of the macroglial cells to BMP7. In this study, we test the hypothesis that BMP7-mediated gliosis is the result of inflammatory signaling from retinal microglia. Adult mice were injected intravitreally with BMP7 and eyes harvested 1, 3, or 7 days postinjection. Some mice were treated with PLX5622 (PLX) to ablate microglia and were subsequently injected with control or BMP7. Processed tissue was analyzed via immunofluorescence, RT-qPCR, or ELISA. In addition, cultures of retinal microglia were treated with vehicle, lipopolysaccharide, or BMP7 to determine the effects of BMP7-isolated cells. Mice injected with BMP7 showed regulation of various inflammatory markers at the RNA level, as well as changes in microglial morphology. Isolated retinal microglia also showed an upregulation of BMP-signaling components following treatment. In vitro treatment of retinal astrocytes with conditioned media from activated microglia upregulated RNA levels of gliosis markers. In the absence of microglia, the mouse retina showed a subdued gliosis and inflammatory response when exposed to BMP7. Gliosis resulting from BMP7 is mediated through an inflammatory response from retinal microglia.

Twitter Demographics

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Mendeley readers

The data shown below were compiled from readership statistics for 42 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 42 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 9 21%
Student > Ph. D. Student 8 19%
Student > Postgraduate 4 10%
Student > Master 4 10%
Student > Doctoral Student 3 7%
Other 8 19%
Unknown 6 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 26%
Neuroscience 9 21%
Agricultural and Biological Sciences 6 14%
Medicine and Dentistry 4 10%
Pharmacology, Toxicology and Pharmaceutical Science 3 7%
Other 3 7%
Unknown 6 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 April 2017.
All research outputs
#8,431,832
of 9,665,907 outputs
Outputs from Journal of Neuroinflammation
#1,049
of 1,229 outputs
Outputs of similar age
#220,846
of 262,849 outputs
Outputs of similar age from Journal of Neuroinflammation
#16
of 19 outputs
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