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Fetal testis organ culture reproduces the dynamics of epigenetic reprogramming in rat gonocytes

Overview of attention for article published in Epigenetics & Chromatin, April 2017
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  • Above-average Attention Score compared to outputs of the same age (54th percentile)
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5 tweeters

Citations

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21 Mendeley
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Title
Fetal testis organ culture reproduces the dynamics of epigenetic reprogramming in rat gonocytes
Published in
Epigenetics & Chromatin, April 2017
DOI 10.1186/s13072-017-0127-3
Pubmed ID
Authors

Arlette Rwigemera, Fabien Joao, Geraldine Delbes

Abstract

Epigenetic reprogramming is a critical step in male germ cell development that occurs during perinatal life. It is characterized by the remodeling of different epigenetic marks such as DNA methylation (5mC) and methylation of histone H3. It has been suggested that endocrine disruptors can affect the male germline epigenome by altering epigenetic reprogramming, but the mechanisms involved are still unknown. We have previously used an organ culture system that maintains the development of the different fetal testis cell types, to evaluate the effects of various endocrine disruptors on gametogenesis and steroidogenesis in the rat. We hypothesize that this culture model can reproduce the epigenetic reprogramming in gonocytes. Our aim was to establish the kinetics of three epigenetic marks throughout perinatal development in rats in vivo and compare them after different culture times. Using immunofluorescence, we showed that H3K4me2 transiently increased in gonocytes at 18.5 days post-coitum (dpc), while H3K4me3 displayed a stable increase in gonocytes from 18.5 dpc until after birth. 5mC progressively increased from 20.5 dpc until after birth. Using GFP-positive gonocytes purified from GCS-EGFP rats, we established the chronology of re-methylation of H19 and Snrpn in rat gonocytes. Most importantly, using testis explanted at 16.5 or 18.5 dpc and cultured for 2-4 days, we demonstrated that the kinetics of changes in H3K4me2, H3K4me3, global DNA methylation and on parental imprints can generally be reproduced ex vivo with the model of organ culture without the addition of serum. This study reveals the chronology of three epigenetic marks (H3K4me2, H3K4me3 and 5mC) and the patterns of methylation of H19 and Snrpn differentially methylated regions in rat gonocytes during perinatal development. Most importantly, our results suggest that the organ culture can reproduce the process of epigenetic reprogramming and can be used to study the impact of environmental chemicals on the establishment of the male germ cell epigenome.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 5%
Unknown 20 95%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 29%
Student > Master 4 19%
Researcher 4 19%
Student > Bachelor 2 10%
Lecturer > Senior Lecturer 1 5%
Other 2 10%
Unknown 2 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 10 48%
Medicine and Dentistry 2 10%
Veterinary Science and Veterinary Medicine 1 5%
Nursing and Health Professions 1 5%
Social Sciences 1 5%
Other 3 14%
Unknown 3 14%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 January 2018.
All research outputs
#6,718,430
of 12,395,352 outputs
Outputs from Epigenetics & Chromatin
#241
of 357 outputs
Outputs of similar age
#113,950
of 262,857 outputs
Outputs of similar age from Epigenetics & Chromatin
#8
of 14 outputs
Altmetric has tracked 12,395,352 research outputs across all sources so far. This one is in the 44th percentile – i.e., 44% of other outputs scored the same or lower than it.
So far Altmetric has tracked 357 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 6.6. This one is in the 29th percentile – i.e., 29% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 262,857 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 54% of its contemporaries.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.