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Production of high-concentration n-caproic acid from lactate through fermentation using a newly isolated Ruminococcaceae bacterium CPB6

Overview of attention for article published in Biotechnology for Biofuels and Bioproducts, April 2017
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  • Above-average Attention Score compared to outputs of the same age and source (60th percentile)

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Title
Production of high-concentration n-caproic acid from lactate through fermentation using a newly isolated Ruminococcaceae bacterium CPB6
Published in
Biotechnology for Biofuels and Bioproducts, April 2017
DOI 10.1186/s13068-017-0788-y
Pubmed ID
Authors

Xiaoyu Zhu, Yan Zhou, Yi Wang, Tingting Wu, Xiangzhen Li, Daping Li, Yong Tao

Abstract

n-Caproic acid (CA), as a medium-chain carboxylic acid, is a valuable chemical feedstock for various industrial applications. The fermentative production of CA from renewable carbon sources has attracted a lot of attentions. Lactate is a significant intermediate waste in the anaerobic breakdown of carbohydrates that comprise 18-70% of the chemical oxygen demand (COD) in municipal and some industrial wastewaters. Recently, researchers (including our own group) reported the CA production using lactate as electron donor with newly identified microbiome systems. However, within such processes, it was hard to determine whether the CA production was completed by a single strain or by the co-metabolism of different microorganisms. Here, we report the CA production using lactate as electron donor using the strain CPB6, which we isolated from a microbiome for CA production as described previously. Strain CPB6 is affiliated with Clostridium cluster IV of the family of Ruminococcaceae based on 16S rRNA gene sequence analysis. The strain prefers acidic initial pH condition (pH 5.0-6.5), and the temperature ranging from 30 to 40 °C for CA production. In a fed-batch fermentation with non-sterilized lactate-containing organic wastewater as feedstock, strain CPB6 produced 16.6 g/L CA (from 45.1 g/L lactate) with a maximum productivity of 5.29 g/L/day. Enzyme assays with crude cell extract showed that CPB6 can metabolize acetate and butyryl-CoA to produce n-butyric acid, and acetate/n-butyrate and caproyl-CoA to produce CA, respectively. This study demonstrated that high concentration of CA production can be obtained by a newly isolated pure culture CPB6. This strain can be employed as a powerful workhorse for high-efficient CA recovery from lactate-containing waste streams. Our preliminary investigation suggested that the CA production from lactate in strain CPB6 might be via the chain elongation pathway of the reverse β-oxidation; the detailed mechanism, however, warrants further investigation using various molecular microbiology techniques.

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X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 191 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 191 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 39 20%
Student > Master 24 13%
Researcher 19 10%
Student > Bachelor 12 6%
Student > Doctoral Student 11 6%
Other 16 8%
Unknown 70 37%
Readers by discipline Count As %
Environmental Science 30 16%
Biochemistry, Genetics and Molecular Biology 19 10%
Engineering 18 9%
Agricultural and Biological Sciences 16 8%
Chemical Engineering 9 5%
Other 18 9%
Unknown 81 42%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 5. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 October 2023.
All research outputs
#7,049,212
of 25,382,440 outputs
Outputs from Biotechnology for Biofuels and Bioproducts
#455
of 1,578 outputs
Outputs of similar age
#104,014
of 323,266 outputs
Outputs of similar age from Biotechnology for Biofuels and Bioproducts
#24
of 64 outputs
Altmetric has tracked 25,382,440 research outputs across all sources so far. This one has received more attention than most of these and is in the 71st percentile.
So far Altmetric has tracked 1,578 research outputs from this source. They receive a mean Attention Score of 4.9. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 323,266 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 64 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 60% of its contemporaries.