Title |
Conjugative transfer of an IncA/C plasmid-borne blaCMY-2gene through genetic re-arrangements with an IncX1 plasmid
|
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Published in |
BMC Microbiology, November 2013
|
DOI | 10.1186/1471-2180-13-264 |
Pubmed ID | |
Authors |
Magdalena Wiesner, Marcos Fernández-Mora, Miguel A Cevallos, Crispín Zavala-Alvarado, Mussaret B Zaidi, Edmundo Calva, Claudia Silva |
Abstract |
Our observation that in the Mexican Salmonella Typhimurium population none of the ST19 and ST213 strains harbored both the Salmonella virulence plasmid (pSTV) and the prevalent IncA/C plasmid (pA/C) led us to hypothesize that restriction to horizontal transfer of these plasmids existed. We designed a conjugation scheme using ST213 strain YU39 as donor of the blaCMY-2 gene (conferring resistance to ceftriaxone; CRO) carried by pA/C, and two E. coli lab strains (DH5α and HB101) and two Typhimurium ST19 strains (SO1 and LT2) carrying pSTV as recipients. The aim of this study was to determine if the genetic background of the different recipient strains affected the transfer frequencies of pA/C. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Colombia | 1 | 2% |
Portugal | 1 | 2% |
Tanzania, United Republic of | 1 | 2% |
Brazil | 1 | 2% |
Unknown | 50 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 17 | 31% |
Student > Ph. D. Student | 14 | 26% |
Student > Master | 4 | 7% |
Student > Bachelor | 3 | 6% |
Professor | 2 | 4% |
Other | 4 | 7% |
Unknown | 10 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 13 | 24% |
Biochemistry, Genetics and Molecular Biology | 11 | 20% |
Immunology and Microbiology | 4 | 7% |
Veterinary Science and Veterinary Medicine | 3 | 6% |
Medicine and Dentistry | 3 | 6% |
Other | 6 | 11% |
Unknown | 14 | 26% |