Title |
Genetic engineering of Yarrowia lipolytica for enhanced production of trans-10, cis-12 conjugated linoleic acid
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Published in |
Microbial Cell Factories, July 2013
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DOI | 10.1186/1475-2859-12-70 |
Pubmed ID | |
Authors |
Baixi Zhang, Haiqin Chen, Min Li, Zhennan Gu, Yuanda Song, Colin Ratledge, Yong Q Chen, Hao Zhang, Wei Chen |
Abstract |
Conjugated linoleic acid (CLA) has been extensively studied for decades because of its health benefits including cancer prevention, anti-atherogenic and anti-obesity effects, and modulation of the immune system. We previously described the production of trans-10, cis-12 CLA in Yarrowia lipolytica by expressing the gene coding for linoleic acid isomerase from Propionibacterium acnes (pai). However the stable strain produced CLA at about 0.08% of dry cell weight (DCW), a level of production which was not high enough for practical applications. The goal of the present study was to enhance production of CLA by genetic engineering of Y. lipolytica strains. |
X Demographics
Geographical breakdown
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 1 | 1% |
Indonesia | 1 | 1% |
France | 1 | 1% |
Austria | 1 | 1% |
Japan | 1 | 1% |
Unknown | 69 | 93% |
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Researcher | 17 | 23% |
Student > Ph. D. Student | 14 | 19% |
Student > Bachelor | 8 | 11% |
Student > Master | 5 | 7% |
Professor | 4 | 5% |
Other | 11 | 15% |
Unknown | 15 | 20% |
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Biochemistry, Genetics and Molecular Biology | 14 | 19% |
Engineering | 4 | 5% |
Medicine and Dentistry | 3 | 4% |
Chemical Engineering | 2 | 3% |
Other | 9 | 12% |
Unknown | 20 | 27% |