Title |
A transcriptomic approach to elucidate the physiological significance of human cytochrome P450 2S1 in bronchial epithelial cells
|
---|---|
Published in |
BMC Genomics, November 2013
|
DOI | 10.1186/1471-2164-14-833 |
Pubmed ID | |
Authors |
Thushara W Madanayake, Ingrid E Lindquist, Nicholas P Devitt, Joann Mudge, Aaron M Rowland |
Abstract |
Cytochrome P450 2S1 (CYP2S1) is an orphan P450 with an unknown biological function. Data from our laboratory and others suggest that CYP2S1 may have an important physiological role in modulating the synthesis and metabolism of bioactive lipids including prostaglandins and retinoids. CYP2S1 expression is elevated in multiple epithelial-derived cancers as well as in the chronic hyperproliferative disease psoriasis. Whether CYP2S1 expression in proliferative disease is protective, detrimental, or neutral to disease progression remains to be determined. Two human bronchial epithelial cells (BEAS-2B) were constructed to represent chronic depletion of CYP2S1 using short-hairpin RNA (shRNA) silencing directed toward the 3'UTR (759) and exon 3 (984) of the CYP2S1 gene and compared with a non-targeting shRNA control (SCRAM). Both CYP2S1 mRNA and protein were depleted by approximately 75% in stable cell lines derived from both targeted shRNA constructs (759 and 984). To elucidate the biological significance of CYP2S1, we analyzed transcriptome alterations in response to CYP2S1 depletion in human lung cells. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Spain | 1 | 3% |
United States | 1 | 3% |
Unknown | 29 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 19% |
Student > Master | 5 | 16% |
Professor > Associate Professor | 4 | 13% |
Student > Ph. D. Student | 4 | 13% |
Student > Bachelor | 4 | 13% |
Other | 4 | 13% |
Unknown | 4 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 5 | 16% |
Biochemistry, Genetics and Molecular Biology | 4 | 13% |
Medicine and Dentistry | 4 | 13% |
Engineering | 3 | 10% |
Immunology and Microbiology | 2 | 6% |
Other | 9 | 29% |
Unknown | 4 | 13% |