Title |
Isothermal DNA amplification coupled to Au-nanoprobes for detection of mutations associated to Rifampicin resistance in Mycobacterium tuberculosis
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Published in |
Journal of Nanobiotechnology, November 2013
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DOI | 10.1186/1477-3155-11-38 |
Pubmed ID | |
Authors |
Bruno Veigas, Pedro Pedrosa, Isabel Couto, Miguel Viveiros, Pedro V Baptista |
Abstract |
Tuberculosis accounted for 8.7 million new cases in 2011 and continues to be one of the leading human infectious diseases. Burdensome is the increasing rate of multi-drug resistant tuberculosis (MDRTB) and the difficulties created for treatment and public health control programs, especially in developing countries. Resistance to rifampicin (RIF), a first line antibiotic, is commonly associated with point mutations within the rpoB gene of Mycobacterium tuberculosis (Mtb) whose detection is considered the best early molecular predictor for MDRTB. Gold nanoparticles functionalized with thiol-modified oligonucleotides (Au-nanoprobes) have shown the potential to provide a rapid and sensitive detection method for Mtb and single base alterations associated with antibiotic resistance, namely in rpoB gene associated to RIF resistance. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 50% |
Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 1 | <1% |
Portugal | 1 | <1% |
Unknown | 114 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 25 | 22% |
Researcher | 25 | 22% |
Student > Master | 12 | 10% |
Other | 9 | 8% |
Student > Bachelor | 9 | 8% |
Other | 20 | 17% |
Unknown | 16 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 28 | 24% |
Biochemistry, Genetics and Molecular Biology | 15 | 13% |
Medicine and Dentistry | 14 | 12% |
Engineering | 7 | 6% |
Materials Science | 4 | 3% |
Other | 30 | 26% |
Unknown | 18 | 16% |