Title |
Accurate detection of subclonal single nucleotide variants in whole genome amplified and pooled cancer samples using HaloPlex target enrichment
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Published in |
BMC Genomics, December 2013
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DOI | 10.1186/1471-2164-14-856 |
Pubmed ID | |
Authors |
Eva C Berglund, Carl Mårten Lindqvist, Shahina Hayat, Elin Övernäs, Niklas Henriksson, Jessica Nordlund, Per Wahlberg, Erik Forestier, Gudmar Lönnerholm, Ann-Christine Syvänen |
Abstract |
Target enrichment and resequencing is a widely used approach for identification of cancer genes and genetic variants associated with diseases. Although cost effective compared to whole genome sequencing, analysis of many samples constitutes a significant cost, which could be reduced by pooling samples before capture. Another limitation to the number of cancer samples that can be analyzed is often the amount of available tumor DNA. We evaluated the performance of whole genome amplified DNA and the power to detect subclonal somatic single nucleotide variants in non-indexed pools of cancer samples using the HaloPlex technology for target enrichment and next generation sequencing. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Sweden | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Denmark | 2 | 3% |
Netherlands | 1 | 1% |
Austria | 1 | 1% |
Sweden | 1 | 1% |
Australia | 1 | 1% |
Spain | 1 | 1% |
United States | 1 | 1% |
Unknown | 60 | 88% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 23 | 34% |
Student > Ph. D. Student | 14 | 21% |
Other | 8 | 12% |
Student > Master | 7 | 10% |
Student > Doctoral Student | 2 | 3% |
Other | 6 | 9% |
Unknown | 8 | 12% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 30 | 44% |
Biochemistry, Genetics and Molecular Biology | 17 | 25% |
Computer Science | 5 | 7% |
Medicine and Dentistry | 3 | 4% |
Mathematics | 1 | 1% |
Other | 0 | 0% |
Unknown | 12 | 18% |