Title |
Aqueous humor polymerase chain reaction in uveitis – utility and safety
|
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Published in |
BMC Ophthalmology, October 2016
|
DOI | 10.1186/s12886-016-0369-z |
Pubmed ID | |
Authors |
Argyrios Chronopoulos, Daniel Roquelaure, Georges Souteyrand, Jörg Dieter Seebach, James Scott Schutz, Gabriele Thumann |
Abstract |
To study the value and safety of aqueous humor polymerase chain reaction (PCR) analysis for Herpes simplex, varicella zoster, cytomegalovirus, Epstein-Barr virus and Toxoplasma gondii in patients with uveitis. Records of 45 consecutive patients with anterior and posterior uveitis who underwent AC paracentesis with PCR were reviewed. The main outcome measure was frequency of PCR positivity. Secondary outcomes were alteration of treatment, safety of paracentesis, and correlation of keratitic precipitates with PCR positivity, RESULTS: The overall PCR positivity was 48.9 % (22/45). Therapy was changed because of the PCR results in 14/45 patients (37.7 %). One patient experienced a paracentesis related complication (1/45, 2.2 %) without long-term sequelae. Aqueous PCR altered the diagnosis and treatment in over a third of our patients and was relatively safe. Aqueous PCR should be considered for uveitis of atypical clinical appearance, recurrent severe uveitis of uncertain etiology, and therapy refractory cases. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Colombia | 1 | 2% |
Unknown | 43 | 98% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 7 | 16% |
Student > Postgraduate | 5 | 11% |
Student > Ph. D. Student | 5 | 11% |
Professor | 5 | 11% |
Student > Bachelor | 5 | 11% |
Other | 14 | 32% |
Unknown | 3 | 7% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 29 | 66% |
Agricultural and Biological Sciences | 4 | 9% |
Biochemistry, Genetics and Molecular Biology | 2 | 5% |
Nursing and Health Professions | 2 | 5% |
Immunology and Microbiology | 2 | 5% |
Other | 3 | 7% |
Unknown | 2 | 5% |