Title |
Over-expression of Toll-like receptor 2 up-regulates heme oxygenase-1 expression and decreases oxidative injury in dairy goats
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Published in |
Journal of Animal Science and Biotechnology, January 2017
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DOI | 10.1186/s40104-016-0136-2 |
Pubmed ID | |
Authors |
Shoulong Deng, Kun Yu, Wuqi Jiang, Yan Li, Shuotian Wang, Zhuo Deng, Yuchang Yao, Baolu Zhang, Guoshi Liu, Yixun Liu, Zhengxing Lian |
Abstract |
Mastitis, an infection caused by Gram-positive bacteria, produces udder inflammation and oxidative injury in milk-producing mammals. Toll-like receptor 2 (TLR2) is important for host recognition of invading Gram-positive microbes. Over-expression of TLR2 in transgenic dairy goats is a useful model for studying various aspects of infection with Gram-positive bacteria, in vivo. We over-expressed TLR2 in transgenic dairy goats. Pam3CSK4, a component of Gram-positive bacteria, triggered the TLR2 signal pathway by stimulating the monocytes-macrophages from the TLR2-positive transgenic goats, and induced over-expression of activator protein-1 (AP-1), phosphatidylinositol 3-kinase (PI3K) and transcription factor nuclear factor kappa B (NF-κB) and inflammation factors downstream of the signal pathway. Compared with wild-type controls, measurements of various oxidative stress-related molecules showed that TLR2, when over-expressed in transgenic goat monocytes-macrophages, resulted in weak lipid damage, high level expression of anti-oxidative stress proteins, and significantly increased mRNA levels of transcription factor NF-E2-related factor-2 (Nrf2) and the downstream gene, heme oxygenase-1 (HO-1). When Pam3CSK4 was used to stimulate ear tissue in vivo the HO-1 protein of the transgenic goats had a relatively high expression level. The results indicate that the oxidative injury in goats over-expressing TLR2 was reduced following Pam3CSK4 stimulation. The underlying mechanism for this reduction was increased expression of the anti-oxidation gene HO-1 by activation of the Nrf2 signal pathway. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 22 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 4 | 18% |
Researcher | 3 | 14% |
Student > Doctoral Student | 2 | 9% |
Professor > Associate Professor | 2 | 9% |
Student > Master | 2 | 9% |
Other | 1 | 5% |
Unknown | 8 | 36% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 5 | 23% |
Veterinary Science and Veterinary Medicine | 4 | 18% |
Agricultural and Biological Sciences | 2 | 9% |
Nursing and Health Professions | 1 | 5% |
Immunology and Microbiology | 1 | 5% |
Other | 1 | 5% |
Unknown | 8 | 36% |