Title |
An improved multiple-locus variable-number of tandem repeat analysis (MLVA) for the fish pathogen Francisella noatunensis using capillary electrophoresis
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Published in |
BMC Veterinary Research, December 2013
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DOI | 10.1186/1746-6148-9-252 |
Pubmed ID | |
Authors |
Samuel Duodu, Xihe Wan, Nora Martinussen Tandstad, Pär Larsson, Kerstin Myrtennäs, Andreas Sjödin, Mats Forsman, Duncan J Colquhoun |
Abstract |
Francisellosis, caused by the bacterium Francisella noatunensis subsp. noatunensis, remains a serious threat to Atlantic cod (Gadhus morhua) farming in Norway and potentially in other countries. As outbreak strains appear clonal in population structure, access to highly discriminatory typing tools is critical for understanding the epidemiology of francisellosis infections in aquaculture. In this study, a simplified multiple-locus variable-number of tandem repeat analysis (MLVA) targeting five highly polymorphic variable number of tandem repeat (VNTR) loci in a single multiplex PCR was developed to rapidly discriminate between outbreak strains. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 50% |
Sweden | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 23 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 9 | 39% |
Unspecified | 2 | 9% |
Researcher | 2 | 9% |
Student > Bachelor | 1 | 4% |
Lecturer > Senior Lecturer | 1 | 4% |
Other | 2 | 9% |
Unknown | 6 | 26% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 7 | 30% |
Immunology and Microbiology | 4 | 17% |
Veterinary Science and Veterinary Medicine | 3 | 13% |
Unspecified | 2 | 9% |
Biochemistry, Genetics and Molecular Biology | 1 | 4% |
Other | 0 | 0% |
Unknown | 6 | 26% |