Title |
Sensitive multiplex PCR assay to differentiate Lyme spirochetes and emerging pathogens Anaplasma phagocytophilum and Babesia microti
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Published in |
BMC Microbiology, December 2013
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DOI | 10.1186/1471-2180-13-295 |
Pubmed ID | |
Authors |
Kamfai Chan, Salvatore AE Marras, Nikhat Parveen |
Abstract |
The infection with Borrelia burgdorferi can result in acute to chronic Lyme disease. In addition, coinfection with tick-borne pathogens, Babesia species and Anaplasma phagocytophilum has been increasing in endemic regions of the USA and Europe. The currently used serological diagnostic tests are often difficult to interpret and, moreover, antibodies against the pathogens persist for a long time making it difficult to confirm the cure of the disease. In addition, these tests cannot be used for diagnosis of early disease state before the adaptive immune response is established. Since nucleic acids of the pathogens do not persist after the cure, DNA-based diagnostic tests are becoming highly useful for detecting infectious diseases. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 20% |
Ireland | 1 | 20% |
Unknown | 3 | 60% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 80% |
Practitioners (doctors, other healthcare professionals) | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Russia | 3 | 3% |
Japan | 1 | 1% |
Unknown | 82 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 28 | 33% |
Student > Ph. D. Student | 10 | 12% |
Student > Master | 7 | 8% |
Student > Doctoral Student | 7 | 8% |
Other | 5 | 6% |
Other | 17 | 20% |
Unknown | 12 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 26 | 30% |
Veterinary Science and Veterinary Medicine | 10 | 12% |
Immunology and Microbiology | 10 | 12% |
Medicine and Dentistry | 9 | 10% |
Biochemistry, Genetics and Molecular Biology | 9 | 10% |
Other | 9 | 10% |
Unknown | 13 | 15% |