↓ Skip to main content

Identification of potential Campylobacter jejuni genes involved in biofilm formation by EZ-Tn5 Transposome mutagenesis

Overview of attention for article published in BMC Research Notes, May 2017
Altmetric Badge

Mentioned by

twitter
2 X users

Citations

dimensions_citation
12 Dimensions

Readers on

mendeley
32 Mendeley
You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output. Click here to find out more.
Title
Identification of potential Campylobacter jejuni genes involved in biofilm formation by EZ-Tn5 Transposome mutagenesis
Published in
BMC Research Notes, May 2017
DOI 10.1186/s13104-017-2504-1
Pubmed ID
Authors

Amy Huei Teen Teh, Sui Mae Lee, Gary A. Dykes

Abstract

Biofilm formation has been suggested to play a role in the survival of Campylobacter jejuni in the environment and contribute to the high incidence of human campylobacteriosis. Molecular studies of biofilm formation by Campylobacter are sparse. We attempted to identify genes that may be involved in biofilm formation in seven C. jejuni strains through construction of mutants using the EZ-Tn5 Transposome system. Only 14 mutants with reduced biofilm formation were obtained, all from one strain of C. jejuni. Three different genes of interest, namely CmeB (synthesis of multidrug efflux system transporter proteins), NusG (transcription termination and anti-termination protein) and a putative transmembrane protein (involved in membrane protein function) were identified. The efficiency of the EZ::TN5 transposon mutagenesis approach was strain dependent and was unable to generate any mutants from most of the strains used. A diverse range of genes may be involved in biofilm formation by C. jejuni. The application of the EZ::TN5 system for construction of mutants in different Campylobacter strains is limited.

X Demographics

X Demographics

The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 32 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 32 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 19%
Student > Ph. D. Student 5 16%
Student > Doctoral Student 3 9%
Student > Master 3 9%
Researcher 2 6%
Other 6 19%
Unknown 7 22%
Readers by discipline Count As %
Agricultural and Biological Sciences 8 25%
Immunology and Microbiology 5 16%
Biochemistry, Genetics and Molecular Biology 5 16%
Environmental Science 1 3%
Veterinary Science and Veterinary Medicine 1 3%
Other 2 6%
Unknown 10 31%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 March 2018.
All research outputs
#18,830,858
of 23,337,345 outputs
Outputs from BMC Research Notes
#3,054
of 4,307 outputs
Outputs of similar age
#237,554
of 311,009 outputs
Outputs of similar age from BMC Research Notes
#39
of 58 outputs
Altmetric has tracked 23,337,345 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,307 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 16th percentile – i.e., 16% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 311,009 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 58 others from the same source and published within six weeks on either side of this one. This one is in the 12th percentile – i.e., 12% of its contemporaries scored the same or lower than it.