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| Title |
Experimental design approach in recombinant protein expression: determining medium composition and induction conditions for expression of pneumolysin from Streptococcus pneumoniae in Escherichia coliand preliminary purification process
|
|---|---|
| Published in |
BMC Biotechnology, January 2014
|
| DOI | 10.1186/1472-6750-14-1 |
| Pubmed ID | |
| Authors |
Guillermo Marini, Mateus Dalcin Luchese, Ana Paula Correa Argondizzo, Ana Carolina Magalhães Andrade de Góes, Ricardo Galler, Tito Lívio Moitinho Alves, Marco Alberto Medeiros, Ariane Leites Larentis |
| Abstract |
Streptococcus pneumoniae (S. pneumoniae) causes several serious diseases including pneumonia, septicemia and meningitis. The World Health Organization estimates that streptococcal pneumonia is the cause of approximately 1.9 million deaths of children under five years of age each year. The large number of serotypes underlying the disease spectrum, which would be reflected in the high production cost of a commercial vaccine effective to protect against all of them and the higher level of amino acid sequence conservation as compared to polysaccharide structure, has prompted us to attempt to use conserved proteins for the development of a simpler vaccine. One of the most prominent proteins is pneumolysin (Ply), present in almost all the serotypes known at the moment, which shows an effective protection against S. pneumoniae infections. |
Mendeley readers
The data shown below were compiled from readership statistics for 120 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| Switzerland | 1 | <1% |
| Unknown | 118 | 98% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 23 | 19% |
| Student > Ph. D. Student | 20 | 17% |
| Researcher | 19 | 16% |
| Student > Bachelor | 18 | 15% |
| Professor | 4 | 3% |
| Other | 13 | 11% |
| Unknown | 23 | 19% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 30 | 25% |
| Biochemistry, Genetics and Molecular Biology | 23 | 19% |
| Medicine and Dentistry | 11 | 9% |
| Engineering | 7 | 6% |
| Chemistry | 6 | 5% |
| Other | 19 | 16% |
| Unknown | 24 | 20% |