Title |
One-step immortalization of primary human airway epithelial cells capable of oncogenic transformation
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Published in |
Cell & Bioscience, November 2016
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DOI | 10.1186/s13578-016-0122-6 |
Pubmed ID | |
Authors |
Jordan L. Smith, Liam C. Lee, Abigail Read, Qiuning Li, Bing Yu, Chih-Shia Lee, Ji Luo |
Abstract |
The ability to transform normal human cells into cancer cells with the introduction of defined genetic alterations is a valuable method for understanding the mechanisms of oncogenesis. Easy establishment of immortalized but non-transformed human cells from various tissues would facilitate these genetic analyses. We report here a simple, one-step immortalization method that involves retroviral vector mediated co-expression of the human telomerase protein and a shRNA targeting the CDKN2A gene locus. We demonstrate that this method could successfully immortalize human small airway epithelial cells while maintaining their chromosomal stability. We further showed that these cells retain p53 activity and can be transformed by the KRAS oncogene. Our method simplifies the immortalization process and is broadly applicable for establishing immortalized epithelial cell lines from primary human tissues for cancer research. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 24 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 25% |
Student > Bachelor | 5 | 21% |
Researcher | 4 | 17% |
Student > Master | 3 | 13% |
Student > Doctoral Student | 1 | 4% |
Other | 1 | 4% |
Unknown | 4 | 17% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 14 | 58% |
Agricultural and Biological Sciences | 2 | 8% |
Immunology and Microbiology | 2 | 8% |
Medicine and Dentistry | 1 | 4% |
Engineering | 1 | 4% |
Other | 0 | 0% |
Unknown | 4 | 17% |