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Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens

Overview of attention for article published in Veterinary Research, January 2014
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  • Good Attention Score compared to outputs of the same age (71st percentile)
  • Good Attention Score compared to outputs of the same age and source (78th percentile)

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Title
Genotyping of a microsatellite locus to differentiate clinical Ostreid herpesvirus 1 specimens
Published in
Veterinary Research, January 2014
DOI 10.1186/1297-9716-45-3
Pubmed ID
Authors

Tristan Renault, Gwenaëlle Tchaleu, Nicole Faury, Pierrick Moreau, Amélie Segarra, Valérie Barbosa-Solomieu, Sylvie Lapègue

Abstract

Ostreid herpesvirus 1 (OsHV-1) is a DNA virus belonging to the Malacoherpesviridae family from the Herpesvirales order. OsHV-1 has been associated with mortality outbreaks in different bivalve species including the Pacific cupped oyster, Crassostrea gigas. Since 2008, massive mortality events have been reported among C. gigas in Europe in relation to the detection of a variant of OsHV-1, called μVar. Since 2009, this variant has been mainly detected in France. These results raise questions about the emergence and the virulence of this variant. The search for association between specific virus genetic markers and clinical symptoms is of great interest and the characterization of the genetic variability of OsHV-1 specimens is an area of growing interest. Determination of nucleotide sequences of PCR-amplified virus DNA fragments has already been used to characterize OsHV-1 specimens and virus variants have thus been described. However, the virus DNA sequencing approach is time-consuming in the high-scale format. Identification and genotyping of highly polymorphic microsatellite loci appear as a suitable approach. The main objective of the present study was the development of a genotyping method in order to characterise clinical OsHV-1 specimens by targeting a particular microsatellite locus located in the ORF4 area. Genotyping results were compared to sequences already available. An excellent correlation was found between the detected genotypes and the corresponding sequences showing that the genotyping approach allowed an accuraté discrimination between virus specimens.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 37 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 37 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 12 32%
Student > Ph. D. Student 7 19%
Student > Doctoral Student 3 8%
Student > Master 3 8%
Professor 2 5%
Other 5 14%
Unknown 5 14%
Readers by discipline Count As %
Agricultural and Biological Sciences 12 32%
Environmental Science 7 19%
Veterinary Science and Veterinary Medicine 4 11%
Biochemistry, Genetics and Molecular Biology 3 8%
Immunology and Microbiology 3 8%
Other 1 3%
Unknown 7 19%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 June 2015.
All research outputs
#7,960,512
of 25,374,917 outputs
Outputs from Veterinary Research
#375
of 1,337 outputs
Outputs of similar age
#87,448
of 319,046 outputs
Outputs of similar age from Veterinary Research
#7
of 33 outputs
Altmetric has tracked 25,374,917 research outputs across all sources so far. This one has received more attention than most of these and is in the 67th percentile.
So far Altmetric has tracked 1,337 research outputs from this source. They receive a mean Attention Score of 5.0. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 319,046 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.