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Ubiquitin-like prokaryotic MoaD as a fusion tag for expression of heterologous proteins in Escherichia coli

Overview of attention for article published in BMC Biotechnology, January 2014
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Title
Ubiquitin-like prokaryotic MoaD as a fusion tag for expression of heterologous proteins in Escherichia coli
Published in
BMC Biotechnology, January 2014
DOI 10.1186/1472-6750-14-5
Pubmed ID
Authors

Sujuan Yuan, Xin Wang, Jian Xu, Zheng Yan, Nan Wang

Abstract

Eukaryotic ubiquitin and SUMO are frequently used as tags to enhance the fusion protein expression in microbial host. They increase the solubility and stability, and protect the peptides from proteolytic degradation due to their stable and highly conserved structures. Few of prokaryotic ubiquitin-like proteins was used as fusion tags except ThiS, which enhances the fusion expression, however, reduces the solubility and stability of the expressed peptides in E. coli. Hence, we investigated if MoaD, a conserved small sulfur carrier in prokaryotes with the similar structure of ubiquitin, could also be used as fusion tag in heterologous expression in E. coli.

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The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 21 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 5 24%
Student > Master 4 19%
Researcher 4 19%
Student > Bachelor 2 10%
Lecturer 1 5%
Other 3 14%
Unknown 2 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 6 29%
Agricultural and Biological Sciences 5 24%
Chemistry 3 14%
Pharmacology, Toxicology and Pharmaceutical Science 1 5%
Computer Science 1 5%
Other 3 14%
Unknown 2 10%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 January 2014.
All research outputs
#18,360,179
of 22,739,983 outputs
Outputs from BMC Biotechnology
#763
of 935 outputs
Outputs of similar age
#228,496
of 305,591 outputs
Outputs of similar age from BMC Biotechnology
#10
of 14 outputs
Altmetric has tracked 22,739,983 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 935 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.7. This one is in the 9th percentile – i.e., 9% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 305,591 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 14 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.