Title |
Expression of the Aspergillus terreus itaconic acid biosynthesis cluster in Aspergillus niger
|
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Published in |
Microbial Cell Factories, January 2014
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DOI | 10.1186/1475-2859-13-11 |
Pubmed ID | |
Authors |
Laura van der Straat, Marloes Vernooij, Marieke Lammers, Willy van den Berg, Tom Schonewille, Jan Cordewener, Ingrid van der Meer, Andries Koops, Leo H de Graaff |
Abstract |
Aspergillus terreus is a natural producer of itaconic acid and is currently used to produce itaconic acid on an industrial scale. The metabolic process for itaconic acid biosynthesis is very similar to the production of citric acid in Aspergillus niger. However, a key enzyme in A. niger, cis-aconitate decarboxylase, is missing. The introduction of the A. terreus cadA gene in A. niger exploits the high level of citric acid production (over 200 g per liter) and theoretically can lead to production levels of over 135 g per liter of itaconic acid in A. niger. Given the potential for higher production levels in A. niger, production of itaconic acid in this host was investigated. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Germany | 3 | 2% |
Netherlands | 1 | <1% |
Indonesia | 1 | <1% |
Brazil | 1 | <1% |
Denmark | 1 | <1% |
China | 1 | <1% |
United States | 1 | <1% |
Unknown | 146 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 31 | 20% |
Researcher | 24 | 15% |
Student > Bachelor | 23 | 15% |
Student > Ph. D. Student | 20 | 13% |
Professor > Associate Professor | 6 | 4% |
Other | 19 | 12% |
Unknown | 32 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 52 | 34% |
Biochemistry, Genetics and Molecular Biology | 37 | 24% |
Chemical Engineering | 10 | 6% |
Engineering | 6 | 4% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 3% |
Other | 8 | 5% |
Unknown | 38 | 25% |