Title |
The global effect of follicle-stimulating hormone and tumour necrosis factor α on gene expression in cultured bovine ovarian granulosa cells
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Published in |
BMC Genomics, January 2014
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DOI | 10.1186/1471-2164-15-72 |
Pubmed ID | |
Authors |
Claire Glister, Nicholas Hatzirodos, Katja Hummitzsch, Philip G Knight, Raymond J Rodgers |
Abstract |
Oocytes mature in ovarian follicles surrounded by granulosa cells. During follicle growth, granulosa cells replicate and secrete hormones, particularly steroids close to ovulation. However, most follicles cease growing and undergo atresia or regression instead of ovulating. To investigate the effects of stimulatory (follicle-stimulating hormone; FSH) and inhibitory (tumour necrosis factor alpha; TNFα) factors on the granulosa cell transcriptome, bovine ovaries were obtained from a local abattoir and pools of granulosa cells were cultured in vitro for six days under defined serum-free conditions with treatments present on days 3-6. Initially dose-response experiments (n = 4) were performed to determine the optimal concentrations of FSH (0.33 ng/ml) and TNFα (10 ng/ml) to be used for the microarray experiments. For array experiments cells were cultured under control conditions, with FSH, with TNFα, or with FSH plus TNFα (n = 4 per group) and RNA was harvested for microarray analyses. |
X Demographics
Geographical breakdown
Country | Count | As % |
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France | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 37 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 16% |
Researcher | 5 | 14% |
Student > Master | 5 | 14% |
Other | 4 | 11% |
Student > Doctoral Student | 3 | 8% |
Other | 9 | 24% |
Unknown | 5 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 10 | 27% |
Veterinary Science and Veterinary Medicine | 5 | 14% |
Medicine and Dentistry | 5 | 14% |
Biochemistry, Genetics and Molecular Biology | 4 | 11% |
Unspecified | 1 | 3% |
Other | 4 | 11% |
Unknown | 8 | 22% |