Title |
A genomic perspective to assessing quality of mass-reared SIT flies used in Mediterranean fruit fly (Ceratitis capitata) eradication in California
|
---|---|
Published in |
BMC Genomics, February 2014
|
DOI | 10.1186/1471-2164-15-98 |
Pubmed ID | |
Authors |
Bernarda Calla, Brian Hall, Shaobin Hou, Scott M Geib |
Abstract |
Temperature sensitive lethal (tsl) mutants of the tephritid C. capitata are used extensively in control programs involving sterile insect technique in California. These flies are artificially reared and treated with ionizing radiation to render males sterile for further release en masse into the field to compete with wild males and disrupt establishment of invasive populations. Recent research suggests establishment of C. capitata in California, despite the fact that over 250 million sterile flies are released weekly as part of the state's preventative program. In this project, genome-level quality assessment was performed, measured as expression differences between the Vienna-7 tsl mutants used in SIT programs and wild flies. RNA-seq was performed to provide a genome-wide map of the messenger RNA populations in C. capitata, and to investigate significant expression changes in Vienna-7 mass reared flies. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
France | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Scientists | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Korea, Republic of | 1 | 1% |
Spain | 1 | 1% |
Unknown | 66 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 16 | 24% |
Researcher | 12 | 18% |
Student > Master | 8 | 12% |
Other | 5 | 7% |
Student > Postgraduate | 4 | 6% |
Other | 10 | 15% |
Unknown | 13 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 39 | 57% |
Biochemistry, Genetics and Molecular Biology | 11 | 16% |
Psychology | 2 | 3% |
Environmental Science | 1 | 1% |
Physics and Astronomy | 1 | 1% |
Other | 1 | 1% |
Unknown | 13 | 19% |