Title |
Heterosubtypic protective immunity against widely divergent influenza subtypes induced by fusion protein 4sM2 in BALB/c mice
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Published in |
Virology Journal, February 2014
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DOI | 10.1186/1743-422x-11-21 |
Pubmed ID | |
Authors |
Mohammed YE Chowdhury, Soo-Kyung Seo, Ho-Jin Moon, Melbourne R Talactac, Jae-Hoon Kim, Min-Eun Park, Hwa-Young Son, Jong-Soo Lee, Chul-Joong Kim |
Abstract |
Regular reformulation of currently available vaccines is necessary due to the unpredictable variability of influenza viruses. Therefore, vaccine based on a highly conserved antigen with capability of induction of effective immune responses could be a potential solution. Influenza matrix protein-2 (M2) is highly conserved across influenza subtypes and a promising candidate for a broadly protective influenza vaccine. For the enhancement of broad protection, four tandem copies of consensus M2 gene containing extracellular (ED) and cytoplasmic (CD) without the trans-membrane domain (TM) reconstituted from H1N1, H5N1 and H9N2 influenza viruses were linked and named as 4sM2. The construct was effectively expressed in Escherichia coli, purified and proteins were used to immunize BALB/c mice. Humoral and cell-mediated immune responses were investigated following administration. |
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Members of the public | 1 | 100% |
Mendeley readers
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Unknown | 17 | 94% |
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Researcher | 4 | 22% |
Student > Master | 4 | 22% |
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Lecturer | 2 | 11% |
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