Title |
S100A8 inhibits PDGF-induced proliferation of airway smooth muscle cells dependent on the receptor for advanced glycation end-products
|
---|---|
Published in |
Biological Research, June 2017
|
DOI | 10.1186/s40659-017-0128-5 |
Pubmed ID | |
Authors |
Yu-Dong Xu, Yu Wang, Lei-Miao Yin, Ling-Ling Peng, Gyoung-Hee Park, Yong-Qing Yang |
Abstract |
Airway remodeling is a key feature of asthma, characterized by increased proliferation of airway smooth muscle cells (ASMCs). S100A8 is a calcium-binding protein with a potential to regulate cell proliferation. Here, the effect of exogenous S100A8 protein on the proliferation of ASMCs induced by platelet-derived growth factor (PDGF) and the underlying molecular mechanism was investigated. Rat ASMCs were cultured with or without a neutralizing antibody to the receptor for advanced glycation end-products (RAGE), a potential receptor for S100A8 protein. Purified recombinant rat S100A8 protein was then added into the cultured cells, and the proliferation of ASMCs induced by PDGF was detected by colorimetric-based WST-8 assay and ampedance-based xCELLigence proliferation assay. The expression levels of RAGE in ASMCs were analyzed using western blotting assay. Results showed that exogenous S100A8 inhibited the PDGF-induced proliferation of rat ASMCs in a dose-dependent manner with the maximal effect at 1 μg/ml in vitro. Furthermore, when ASMCs was pre-treated with anti-RAGE neutralizing antibody, the inhibitory effect of S100A8 on PDGF-induced proliferation was significantly suppressed. In addition, neither the treatment with S100A8 or PDGF alone nor the pre-treatment with rS100A8 followed by PDGF stimulation affected the expression levels of RAGE. Our study demonstrated that S100A8 inhibits PDGF-induced ASMCs proliferation in a manner dependent on membrane receptor RAGE. |
Twitter Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Philippines | 1 | 25% |
Japan | 1 | 25% |
United States | 1 | 25% |
Unknown | 1 | 25% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Science communicators (journalists, bloggers, editors) | 2 | 50% |
Members of the public | 1 | 25% |
Practitioners (doctors, other healthcare professionals) | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 11 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 4 | 36% |
Student > Ph. D. Student | 2 | 18% |
Researcher | 2 | 18% |
Other | 2 | 18% |
Professor | 1 | 9% |
Other | 0 | 0% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 4 | 36% |
Agricultural and Biological Sciences | 2 | 18% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 18% |
Social Sciences | 2 | 18% |
Nursing and Health Professions | 1 | 9% |
Other | 0 | 0% |