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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Identification of the flotillin-1/2 heterocomplex as a target of autoantibodies in bona fide multiple sclerosis
|
|---|---|
| Published in |
Journal of Neuroinflammation, June 2017
|
| DOI | 10.1186/s12974-017-0900-z |
| Pubmed ID | |
| Authors |
S. Hahn, G. Trendelenburg, M. Scharf, Y. Denno, S. Brakopp, B. Teegen, C. Probst, K. P. Wandinger, M. Buttmann, A. Haarmann, F. Szabados, M. vom Dahl, T. Kümpfel, P. Eichhorn, H. Gold, F. Paul, S. Jarius, N. Melzer, W. Stöcker, L. Komorowski |
| Abstract |
Autoantibodies, in particular those against aquaporin-4 and myelin-oligodendrocyte glycoprotein (MOG), aid as biomarkers in the differential diagnosis of demyelination. Here, we report on discovery of autoantibodies against flotillin in patients with multiple sclerosis (MS). The target antigen was identified by histo-immunoprecipitation using the patients' sera and cryosections of rat or pig cerebellum combined with mass spectrometrical analysis. Correct identification was ascertained by indirect immunofluorescence and neutralization tests using the target antigens recombinantly expressed in HEK293 cells. Serum and CSF of the index patient produced a fine-granular IgG indirect immunofluorescence staining of the hippocampal and cerebellar molecular layers. Flotillin-1 and flotillin-2 were identified as target autoantigens. They also reacted with recombinant human flotillin-1/2 co-expressed in HEK293 cells, but not with the individual flotillins in fixed- and live-cell assays. Moreover, neutralization using flotillin-1/2, but not the single flotillins, abolished the tissue reactivity of patient serum. Screening of 521 patients, for whom anti-aquaporin-4 testing was requested and negative, revealed 8 additional patients with anti-flotillin-1/2 autoantibodies. All eight were negative for anti-MOG. Six patients ex post fulfilled the revised McDonald criteria for MS. Vice versa, screening of 538 MS sera revealed anti-flotillin-1/2 autoantibodies in eight patients. The autoantibodies were not found in a cohort of 67 patients with other neural autoantibody-associated syndromes and in 444 healthy blood donors. Autoantibodies against the flotillin-1/2 heterocomplex, a peripheral membrane protein that is involved in axon outgrowth and regeneration of the optic nerve, are present in 1-2% of patients with bona fide MS. |
X Demographics
The data shown below were collected from the profiles of 5 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 2 | 40% |
| Germany | 1 | 20% |
| United Kingdom | 1 | 20% |
| Unknown | 1 | 20% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 3 | 60% |
| Practitioners (doctors, other healthcare professionals) | 2 | 40% |
Mendeley readers
The data shown below were compiled from readership statistics for 44 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 44 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 6 | 14% |
| Other | 5 | 11% |
| Researcher | 5 | 11% |
| Student > Bachelor | 4 | 9% |
| Student > Master | 4 | 9% |
| Other | 8 | 18% |
| Unknown | 12 | 27% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 9 | 20% |
| Medicine and Dentistry | 5 | 11% |
| Agricultural and Biological Sciences | 3 | 7% |
| Biochemistry, Genetics and Molecular Biology | 3 | 7% |
| Immunology and Microbiology | 2 | 5% |
| Other | 4 | 9% |
| Unknown | 18 | 41% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 July 2017.
All research outputs
#4,560,505
of 22,982,639 outputs
Outputs from Journal of Neuroinflammation
#939
of 2,653 outputs
Outputs of similar age
#80,652
of 316,289 outputs
Outputs of similar age from Journal of Neuroinflammation
#12
of 46 outputs
Altmetric has tracked 22,982,639 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 2,653 research outputs from this source. They typically receive more attention than average, with a mean Attention Score of 7.6. This one has gotten more attention than average, scoring higher than 64% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 316,289 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 74% of its contemporaries.
We're also able to compare this research output to 46 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.