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X Demographics
Mendeley readers
Attention Score in Context
| Title |
Incorporation of non-natural nucleotides into template-switching oligonucleotides reduces background and improves cDNA synthesis from very small RNA samples
|
|---|---|
| Published in |
BMC Genomics, July 2010
|
| DOI | 10.1186/1471-2164-11-413 |
| Pubmed ID | |
| Authors |
Jeremy Kapteyn, Ruifeng He, Eric T McDowell, David R Gang |
| Abstract |
The template switching PCR (TS-PCR) method of cDNA synthesis represents one of the most straightforward approaches to generating full length cDNA for sequencing efforts. However, when applied to very small RNA samples, such as those obtained from tens or hundreds of cells, this approach leads to high background and low cDNA yield due to concatamerization of the TS oligo. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Japan | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 186 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 6 | 3% |
| United Kingdom | 2 | 1% |
| South Africa | 1 | <1% |
| Japan | 1 | <1% |
| Germany | 1 | <1% |
| Unknown | 175 | 94% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 59 | 32% |
| Student > Ph. D. Student | 36 | 19% |
| Student > Bachelor | 14 | 8% |
| Student > Master | 12 | 6% |
| Other | 10 | 5% |
| Other | 29 | 16% |
| Unknown | 26 | 14% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 88 | 47% |
| Biochemistry, Genetics and Molecular Biology | 40 | 22% |
| Medicine and Dentistry | 10 | 5% |
| Immunology and Microbiology | 6 | 3% |
| Neuroscience | 3 | 2% |
| Other | 10 | 5% |
| Unknown | 29 | 16% |
Attention Score in Context
This research output has an Altmetric Attention Score of 7. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 January 2024.
All research outputs
#4,320,093
of 23,427,600 outputs
Outputs from BMC Genomics
#1,777
of 10,766 outputs
Outputs of similar age
#17,568
of 95,304 outputs
Outputs of similar age from BMC Genomics
#4
of 33 outputs
Altmetric has tracked 23,427,600 research outputs across all sources so far. Compared to these this one has done well and is in the 80th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 10,766 research outputs from this source. They receive a mean Attention Score of 4.7. This one has done well, scoring higher than 82% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 95,304 tracked outputs that were published within six weeks on either side of this one in any source. This one has done well, scoring higher than 80% of its contemporaries.
We're also able to compare this research output to 33 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 84% of its contemporaries.