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Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium

Overview of attention for article published in Microbial Cell Factories, February 2014
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Title
Global transcriptomic analysis of an engineered Escherichia coli strain lacking the phosphoenolpyruvate: carbohydrate phosphotransferase system during shikimic acid production in rich culture medium
Published in
Microbial Cell Factories, February 2014
DOI 10.1186/1475-2859-13-28
Pubmed ID
Authors

Larisa Cortés-Tolalpa, Rosa María Gutiérrez-Ríos, Luz María Martínez, Ramón de Anda, Guillermo Gosset, Francisco Bolívar, Adelfo Escalante

Abstract

Efficient production of SA in Escherichia coli has been achieved by modifying key genes of the central carbon metabolism and SA pathway, resulting in overproducing strains grown in batch- or fed-batch-fermentor cultures using a complex broth including glucose and YE. In this study, we performed a GTA to identify those genes significantly upregulated in an engineered E. coli strain, PB12.SA22, in mid EXP (5 h), early STA (STA1, 9 h), and late STA (STA2, 44 h) phases, grown in complex fermentation broth in batch-fermentor cultures.

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Mendeley readers

The data shown below were compiled from readership statistics for 39 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Mexico 2 5%
China 1 3%
Unknown 36 92%

Demographic breakdown

Readers by professional status Count As %
Researcher 9 23%
Student > Ph. D. Student 9 23%
Student > Master 8 21%
Student > Bachelor 2 5%
Other 2 5%
Other 5 13%
Unknown 4 10%
Readers by discipline Count As %
Agricultural and Biological Sciences 16 41%
Biochemistry, Genetics and Molecular Biology 10 26%
Chemistry 2 5%
Unspecified 1 3%
Psychology 1 3%
Other 3 8%
Unknown 6 15%