You are seeing a free-to-access but limited selection of the activity Altmetric has collected about this research output.
Click here to find out more.
Mendeley readers
| Title |
The effect of chlormadinone acetate on odontogenic differentiation of human dental pulp cells: in vitro study
|
|---|---|
| Published in |
BMC Oral Health, May 2017
|
| DOI | 10.1186/s12903-017-0379-0 |
| Pubmed ID | |
| Authors |
Se-Min Kim, Bin-Na Lee, Jeong-Tae Koh, Hoon-Sang Chang, In-Nam Hwang, Won-Mann Oh, Kyung-San Min, Yun-Chan Hwang |
| Abstract |
Chlormadinone acetate (CMA) is a derivative of progesterone and is used as an oral contraceptive. The aim of this study was to investigate the effects of CMA on odontogenic differentiation and mineralization of human dental pulp cells (hDPCs) and related signaling pathways. Cell viability was determined by the water-soluble tetrazolium (WST)-1 assay. Odontogenic differentiation of hDPCs was evaluated by real-time polymerase chain reaction using odontogenic marker genes, such as alkaline phosphatase (ALP), osteocalcin (OCN), dentin sialophosphoprotein (DSPP), and dentin matrix protein-1 (DMP-1). Mineralization of hDPCs was evaluated by ALP staining and alizarin red staining. The extracellular signal-regulated kinase (ERK) pathway was examined by Western blot analysis. There was no statistically significant difference in cell viability between the control and CMA-treated groups. Our analysis of odontogenic marker genes indicated that CMA enhanced the expression of those genes. CMA-treated hDPCs showed increased ALP activity and formation of mineralized nodules, compared with control-treated cells. In addition, CMA stimulation resulted in phosphorylation of ERK and resulted in inhibition of downstream molecules by the ERK inhibitor U0126. These findings suggest that CMA improves odontogenic differentiation and mineralization of hDPCs through the ERK signaling pathway. |
Mendeley readers
The data shown below were compiled from readership statistics for 12 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 12 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 2 | 17% |
| Researcher | 2 | 17% |
| Professor | 1 | 8% |
| Librarian | 1 | 8% |
| Student > Doctoral Student | 1 | 8% |
| Other | 1 | 8% |
| Unknown | 4 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 6 | 50% |
| Biochemistry, Genetics and Molecular Biology | 2 | 17% |
| Unknown | 4 | 33% |