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Bacillus thuringiensis subsp. kurstaki HD1 as a factory to synthesize alkali-labile ChiA74∆sp chitinase inclusions, Cry crystals and spores for applied use

Overview of attention for article published in Microbial Cell Factories, January 2014
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Title
Bacillus thuringiensis subsp. kurstaki HD1 as a factory to synthesize alkali-labile ChiA74∆sp chitinase inclusions, Cry crystals and spores for applied use
Published in
Microbial Cell Factories, January 2014
DOI 10.1186/1475-2859-13-15
Pubmed ID
Authors

José Eleazar Barboza-Corona, Jorge Luis Delgadillo-Ángeles, José Cristóbal Castañeda-Ramírez, Uriel Eleazar Barboza-Pérez, Luz Edith Casados-Vázquez, Dennis K Bideshi, Ma Cristina del Rincón-Castro

Abstract

The endochitinase ChiA74 is a soluble secreted enzyme produced by Bacillus thuringiensis that synergizes the entomotoxigenecity of Cry proteins that accumulate as intracellular crystalline inclusion during sporulation. The purpose of this study was to produce alkaline-soluble ChiA74∆sp inclusions in B. thuringiensis, and to determine its effect on Cry crystal production, sporulation and toxicity to an important agronomical insect, Manduca sexta. To this end we deleted the secretion signal peptide-coding sequence of chiA74 (i.e. chiA74∆sp) and expressed it under its native promoter (pEHchiA74∆sp) or strong chimeric sporulation-dependent cytA-p/STAB-SD promoter (pEBchiA74∆sp) in Escherichia coli, acrystalliferous B. thuringiensis (4Q7) and B. thuringiensis HD1.

X Demographics

X Demographics

The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 45 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 10 22%
Researcher 8 18%
Student > Ph. D. Student 7 16%
Student > Doctoral Student 4 9%
Student > Bachelor 3 7%
Other 7 16%
Unknown 6 13%
Readers by discipline Count As %
Agricultural and Biological Sciences 19 42%
Biochemistry, Genetics and Molecular Biology 12 27%
Immunology and Microbiology 3 7%
Environmental Science 2 4%
Business, Management and Accounting 1 2%
Other 1 2%
Unknown 7 16%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 June 2014.
All research outputs
#14,255,756
of 25,373,627 outputs
Outputs from Microbial Cell Factories
#871
of 1,823 outputs
Outputs of similar age
#166,183
of 320,960 outputs
Outputs of similar age from Microbial Cell Factories
#23
of 35 outputs
Altmetric has tracked 25,373,627 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,823 research outputs from this source. They receive a mean Attention Score of 4.9. This one has gotten more attention than average, scoring higher than 52% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 320,960 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 48th percentile – i.e., 48% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 35 others from the same source and published within six weeks on either side of this one. This one is in the 34th percentile – i.e., 34% of its contemporaries scored the same or lower than it.