Title |
Enrichment of lung microbiome with supraglottic taxa is associated with increased pulmonary inflammation
|
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Published in |
Microbiome, July 2013
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DOI | 10.1186/2049-2618-1-19 |
Pubmed ID | |
Authors |
Leopoldo N Segal, Alexander V Alekseyenko, Jose C Clemente, Rohan Kulkarni, Benjamin Wu, Hao Chen, Kenneth I Berger, Roberta M Goldring, William N Rom, Martin J Blaser, Michael D Weiden |
Abstract |
The lung microbiome of healthy individuals frequently harbors oral organisms. Despite evidence that microaspiration is commonly associated with smoking-related lung diseases, the effects of lung microbiome enrichment with upper airway taxa on inflammation has not been studied. We hypothesize that the presence of oral microorganisms in the lung microbiome is associated with enhanced pulmonary inflammation. To test this, we sampled bronchoalveolar lavage (BAL) from the lower airways of 29 asymptomatic subjects (nine never-smokers, 14 former-smokers, and six current-smokers). We quantified, amplified, and sequenced 16S rRNA genes from BAL samples by qPCR and 454 sequencing. Pulmonary inflammation was assessed by exhaled nitric oxide (eNO), BAL lymphocytes, and neutrophils. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Mexico | 1 | 25% |
Cameroon | 1 | 25% |
United States | 1 | 25% |
Unknown | 1 | 25% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 3 | 75% |
Members of the public | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 5 | 2% |
Germany | 1 | <1% |
Korea, Republic of | 1 | <1% |
Canada | 1 | <1% |
Brazil | 1 | <1% |
Thailand | 1 | <1% |
Russia | 1 | <1% |
Unknown | 291 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 63 | 21% |
Researcher | 60 | 20% |
Student > Master | 40 | 13% |
Student > Bachelor | 30 | 10% |
Student > Doctoral Student | 17 | 6% |
Other | 37 | 12% |
Unknown | 55 | 18% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 71 | 24% |
Biochemistry, Genetics and Molecular Biology | 49 | 16% |
Medicine and Dentistry | 46 | 15% |
Immunology and Microbiology | 43 | 14% |
Nursing and Health Professions | 4 | 1% |
Other | 23 | 8% |
Unknown | 66 | 22% |