Title |
A transcriptomic reporter assay employing neutrophils to measure immunogenic activity of septic patients’ plasma
|
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Published in |
Journal of Translational Medicine, March 2014
|
DOI | 10.1186/1479-5876-12-65 |
Pubmed ID | |
Authors |
Prasong Khaenam, Darawan Rinchai, Matthew C Altman, Laurent Chiche, Surachat Buddhisa, Chidchamai Kewcharoenwong, Duangchan Suwannasaen, Michael Mason, Elizabeth Whalen, Scott Presnell, Wattanachai Susaengrat, Kimberly O’Brien, Quynh-Ahn Nguyen, Vivian Gersuk, Peter S Linsley, Ganjana Lertmemongkolchai, Damien Chaussabel |
Abstract |
There are diverse molecules present in blood plasma that regulate immune functions and also present a potential source of disease biomarkers and therapeutic targets. Genome-wide profiling has become a powerful method for assessing immune responses on a systems scale, but technologies that can measure the plasma proteome still face considerable challenges. An alternative approach to direct proteome assessment is to measure transcriptome responses in reporter cells exposed in vitro to plasma. In this report we describe such a "transcriptomic reporter assay" to assess plasma from patients with sepsis, which is a common and severe systemic infectious process for which physicians lack efficient diagnostic or prognostic markers. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 67% |
Science communicators (journalists, bloggers, editors) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 4% |
Sweden | 1 | 2% |
Unknown | 46 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 13 | 27% |
Student > Ph. D. Student | 5 | 10% |
Student > Doctoral Student | 4 | 8% |
Student > Bachelor | 3 | 6% |
Other | 3 | 6% |
Other | 11 | 22% |
Unknown | 10 | 20% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 14 | 29% |
Agricultural and Biological Sciences | 11 | 22% |
Biochemistry, Genetics and Molecular Biology | 6 | 12% |
Immunology and Microbiology | 5 | 10% |
Unspecified | 1 | 2% |
Other | 2 | 4% |
Unknown | 10 | 20% |