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Isolation, speciation and antifungal susceptibility testing of Candida isolates from various clinical specimens at a tertiary care hospital, Nepal

Overview of attention for article published in BMC Research Notes, June 2017
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Title
Isolation, speciation and antifungal susceptibility testing of Candida isolates from various clinical specimens at a tertiary care hospital, Nepal
Published in
BMC Research Notes, June 2017
DOI 10.1186/s13104-017-2547-3
Pubmed ID
Authors

Sundar Khadka, Jeevan Bahadur Sherchand, Bharat Mani Pokhrel, Keshab Parajuli, Shyam Kumar Mishra, Sangita Sharma, Niranjan Shah, Hari Prasad Kattel, Subhash Dhital, Sulochana Khatiwada, Narayan Parajuli, Manoj Pradhan, Basista Prasad Rijal

Abstract

Candida species are responsible for various clinical infections ranging from mucocutaneous infection to life threatening invasive diseases along with increased resistance to antifungal drugs has made a serious concern. Resistance to antifungal agents has increased during the last decade. Thus, identification of Candida up to species level and its antifungal susceptibility testing has a paramount significance in the management of Candidal infections. The aim of the study was to speciate Candida species and to determine antifungal susceptibility pattern of Candida species to antifungal agents. A total of 100 consecutive Candida species were isolated from 1248 clinical specimens over 7 months period. Growths on Sabouraud dextrose agar were evaluated for colony appearance, macroscopic examination, Gram staining, germ tube test and urea hydrolysis test. Further, they were processed for Candida speciation on CHROMagar. Antifungal susceptibility testing was performed as recommended by Clinical and Laboratory Standards Institute (CLSI) M44-A document. Out of 100 Candida isolates, Candida albicans (56%) was the most common species. Among the non-albicans Candida species, Candida tropicalis (20%) was the predominant isolate followed by Candida glabrata (14%). Regarding antifungal susceptibility pattern, Candida species were more susceptible to clotrimazole (82%) followed by fluconazole (64%) and miconazole (44%). Candida albicans was the predominant species responsible for various Candidal infections. Among commonly used antifungal drugs clotrimazole, miconazole and fluconazole were most effective.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 106 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 106 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 18 17%
Researcher 12 11%
Student > Bachelor 9 8%
Other 6 6%
Student > Postgraduate 6 6%
Other 19 18%
Unknown 36 34%
Readers by discipline Count As %
Immunology and Microbiology 18 17%
Medicine and Dentistry 13 12%
Biochemistry, Genetics and Molecular Biology 9 8%
Agricultural and Biological Sciences 9 8%
Pharmacology, Toxicology and Pharmaceutical Science 3 3%
Other 13 12%
Unknown 41 39%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 26 July 2017.
All research outputs
#15,469,838
of 22,988,380 outputs
Outputs from BMC Research Notes
#2,334
of 4,284 outputs
Outputs of similar age
#198,567
of 315,945 outputs
Outputs of similar age from BMC Research Notes
#58
of 115 outputs
Altmetric has tracked 22,988,380 research outputs across all sources so far. This one is in the 22nd percentile – i.e., 22% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,284 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.5. This one is in the 33rd percentile – i.e., 33% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 315,945 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 28th percentile – i.e., 28% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 115 others from the same source and published within six weeks on either side of this one. This one is in the 38th percentile – i.e., 38% of its contemporaries scored the same or lower than it.