Title |
Imperfect centered miRNA binding sites are common and can mediate repression of target mRNAs
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Published in |
Genome Biology, March 2014
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DOI | 10.1186/gb-2014-15-3-r51 |
Pubmed ID | |
Authors |
Hilary C Martin, Shivangi Wani, Anita L Steptoe, Keerthana Krishnan, Katia Nones, Ehsan Nourbakhsh, Alexander Vlassov, Sean M Grimmond, Nicole Cloonan |
Abstract |
MicroRNAs (miRNAs) bind to mRNAs and target them for translational inhibition or transcriptional degradation. It is thought that most miRNA-mRNA interactions involve the seed region at the 5' end of the miRNA. The importance of seed sites is supported by experimental evidence, although there is growing interest in interactions mediated by the central region of the miRNA, termed centered sites. To investigate the prevalence of these interactions, we apply a biotin pull-down method to determine the direct targets of ten human miRNAs, including four isomiRs that share centered sites, but not seeds, with their canonical partner miRNAs. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
United Kingdom | 3 | 18% |
United States | 2 | 12% |
Turkey | 1 | 6% |
Mexico | 1 | 6% |
India | 1 | 6% |
Singapore | 1 | 6% |
Canada | 1 | 6% |
Japan | 1 | 6% |
France | 1 | 6% |
Other | 1 | 6% |
Unknown | 4 | 24% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 11 | 65% |
Scientists | 5 | 29% |
Science communicators (journalists, bloggers, editors) | 1 | 6% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Denmark | 2 | <1% |
United States | 2 | <1% |
Spain | 2 | <1% |
Australia | 1 | <1% |
Brazil | 1 | <1% |
Italy | 1 | <1% |
Mexico | 1 | <1% |
Germany | 1 | <1% |
Malaysia | 1 | <1% |
Other | 1 | <1% |
Unknown | 205 | 94% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 63 | 29% |
Researcher | 36 | 17% |
Student > Master | 26 | 12% |
Student > Bachelor | 21 | 10% |
Student > Doctoral Student | 17 | 8% |
Other | 35 | 16% |
Unknown | 20 | 9% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 91 | 42% |
Biochemistry, Genetics and Molecular Biology | 50 | 23% |
Medicine and Dentistry | 16 | 7% |
Computer Science | 13 | 6% |
Immunology and Microbiology | 5 | 2% |
Other | 15 | 7% |
Unknown | 28 | 13% |