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Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays

Overview of attention for article published in BMC Research Notes, November 2013
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Title
Validation of miRNA-mRNA interactions by electrophoretic mobility shift assays
Published in
BMC Research Notes, November 2013
DOI 10.1186/1756-0500-6-454
Pubmed ID
Authors

Anna Solé, Núria Mencia, Xenia Villalobos, Véronique Noé, Carlos J Ciudad

Abstract

MicroRNAs are small non-coding RNAs involved in gene expression regulation by targeting specific regions in the 3'-UTR of the mRNA of their target genes. This binding leads to a decrease in the protein levels of such genes either by mRNA degradation or mRNA destabilization and translation inhibition. The interaction between a miRNA and its target mRNAs is usually studied by co-transfection of a reporter expression vector containing the 3'-UTR region of the mRNA and an inhibitory or precursor molecule for the miRNA. This approach, however, does not measure the direct and physical interaction between a miRNA and a specific mRNA.

Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 28 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 10 36%
Professor 4 14%
Student > Bachelor 3 11%
Researcher 3 11%
Student > Doctoral Student 2 7%
Other 4 14%
Unknown 2 7%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 12 43%
Agricultural and Biological Sciences 9 32%
Chemistry 3 11%
Unspecified 1 4%
Medicine and Dentistry 1 4%
Other 0 0%
Unknown 2 7%