Title |
Mathematically optimized cryoprotectant equilibration procedures for cryopreservation of human oocytes
|
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Published in |
Theoretical Biology and Medical Modelling, March 2014
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DOI | 10.1186/1742-4682-11-13 |
Pubmed ID | |
Authors |
Allyson Fry Davidson, James D Benson, Adam Z Higgins |
Abstract |
Simple and effective cryopreservation of human oocytes would have an enormous impact on the financial and ethical constraints of human assisted reproduction. Recently, studies have demonstrated the potential for cryopreservation in an ice-free glassy state by equilibrating oocytes with high concentrations of cryoprotectants (CPAs) and rapidly cooling to liquid nitrogen temperatures. A major difficulty with this approach is that the high concentrations required for the avoidance of crystal formation (vitrification) also increase the risk of osmotic and toxic damage. We recently described a mathematical optimization approach for designing CPA equilibration procedures that avoid osmotic damage and minimize toxicity, and we presented optimized procedures for human oocytes involving continuous changes in solution composition. |
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