Title |
The use of microbead-based spoligotyping for Mycobacterium tuberculosis complex to evaluate the quality of the conventional method: Providing guidelines for Quality Assurance when working on membranes
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Published in |
BMC Infectious Diseases, April 2011
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DOI | 10.1186/1471-2334-11-110 |
Pubmed ID | |
Authors |
Edgar Abadia, Jian Zhang, Viviana Ritacco, Kristin Kremer, Raymond Ruimy, Leen Rigouts, Harrison Magdinier Gomes, Atiná Ribeiro Elias, Maryse Fauville-Dufaux, Karolien Stoffels, Voahangy Rasolofo-Razanamparany, Darío Garcia de Viedma, Marta Herranz, Sahal Al-Hajoj, Nalin Rastogi, Carlo Garzelli, Enrico Tortoli, Philip N Suffys, Dick van Soolingen, Guislaine Refrégier, Christophe Sola |
Abstract |
The classical spoligotyping technique, relying on membrane reverse line-blot hybridization of the spacers of the Mycobacterium tuberculosis CRISPR locus, is used world-wide (598 references in Pubmed on April 8th, 2011). However, until now no inter-laboratory quality control study had been undertaken to validate this technique. We analyzed the quality of membrane-based spoligotyping by comparing it to the recently introduced and highly robust microbead-based spoligotyping. Nine hundred and twenty-seven isolates were analyzed totaling 39,861 data points. Samples were received from 11 international laboratories with a worldwide distribution. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Colombia | 1 | 1% |
Zimbabwe | 1 | 1% |
Zambia | 1 | 1% |
Brazil | 1 | 1% |
United Kingdom | 1 | 1% |
United States | 1 | 1% |
Unknown | 74 | 93% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 18 | 23% |
Student > Ph. D. Student | 9 | 11% |
Student > Master | 8 | 10% |
Professor > Associate Professor | 7 | 9% |
Student > Doctoral Student | 5 | 6% |
Other | 16 | 20% |
Unknown | 17 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 19 | 24% |
Medicine and Dentistry | 14 | 18% |
Biochemistry, Genetics and Molecular Biology | 12 | 15% |
Immunology and Microbiology | 10 | 13% |
Social Sciences | 3 | 4% |
Other | 1 | 1% |
Unknown | 21 | 26% |