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Profiling of the perturbed metabolomic state of mouse spleen during acute and chronic toxoplasmosis

Overview of attention for article published in Parasites & Vectors, July 2017
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Title
Profiling of the perturbed metabolomic state of mouse spleen during acute and chronic toxoplasmosis
Published in
Parasites & Vectors, July 2017
DOI 10.1186/s13071-017-2282-6
Pubmed ID
Authors

Xiao-Qing Chen, Chun-Xue Zhou, Hany M. Elsheikha, Shuai He, Gui-Xue Hu, Xing-Quan Zhu

Abstract

Toxoplasma gondii, a common opportunistic protozoan, is a leading cause of illness and mortality among immunosuppressed individuals and during congenital infections. Current therapeutic strategies for toxoplasmosis are not fully effective at curtailing disease progression in these cases. Given the parasite ability to influence host immunity and metabolism, understanding of the metabolic alterations in the host's immune organs during T. gondii infection may enhance the understanding of the molecular mechanisms that define the pathophysiology of T. gondii infection. We investigated the global metabolic changes in the spleen of BALB/c mice at early and late stage of infection with T. gondii using LC-MS/MS-based metabolomics. Multivariate data analysis methods, principal components analysis (PCA) and partial least squares discriminant analysis (PLS-DA), were used to identify metabolites that are influenced by T. gondii infection. Multivariate analyses clearly separated the metabolites of spleen of infected and control mice. A total of 132 differential metabolites were identified, 23 metabolites from acutely infected versus control mice and 109 metabolites from chronically infected versus control mice. Lipids, hormones, lactones, acids, peptides, antibiotics, alkaloids and natural toxins were the most influenced chemical groups. There were 12 shared differential metabolites between acutely infected versus control mice and chronically infected versus control mice, of which 4,4-Dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol was significantly upregulated and ubiquinone-8 was significantly downregulated. Major perturbed metabolic pathways included primary bile acid biosynthesis, steroid hormone biosynthesis, biotin metabolism, and steroid biosynthesis, with arachidonic acid metabolism being the most significantly impacted pathway. These metabolic changes suggest a multifactorial nature of the immunometabolic responses of mouse spleen to T. gondii infection. This study demonstrated that T. gondii infection can cause significant metabolomic alterations in the spleen of infected mice. These findings provide new insights into the molecular mechanisms that underpin the pathogenesis of T. gondii infection.

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Mendeley readers

The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 24 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 6 25%
Professor 3 13%
Researcher 3 13%
Student > Master 3 13%
Student > Bachelor 2 8%
Other 5 21%
Unknown 2 8%
Readers by discipline Count As %
Agricultural and Biological Sciences 6 25%
Veterinary Science and Veterinary Medicine 4 17%
Biochemistry, Genetics and Molecular Biology 4 17%
Immunology and Microbiology 3 13%
Environmental Science 2 8%
Other 2 8%
Unknown 3 13%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 July 2017.
All research outputs
#18,562,247
of 22,990,068 outputs
Outputs from Parasites & Vectors
#4,254
of 5,494 outputs
Outputs of similar age
#241,103
of 314,952 outputs
Outputs of similar age from Parasites & Vectors
#110
of 124 outputs
Altmetric has tracked 22,990,068 research outputs across all sources so far. This one is in the 11th percentile – i.e., 11% of other outputs scored the same or lower than it.
So far Altmetric has tracked 5,494 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 5.7. This one is in the 11th percentile – i.e., 11% of its peers scored the same or lower than it.
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We're also able to compare this research output to 124 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.