Title |
LabKey Server NAb: A tool for analyzing, visualizing and sharing results from neutralizing antibody assays
|
---|---|
Published in |
BMC Immunology, May 2011
|
DOI | 10.1186/1471-2172-12-33 |
Pubmed ID | |
Authors |
Britt Piehler, Elizabeth K Nelson, Josh Eckels, Sarah Ramsay, Karl Lum, Blake Wood, Kelli M Greene, Hongmei Gao, Michael S Seaman, David C Montefiori, Mark Igra |
Abstract |
Multiple types of assays allow sensitive detection of virus-specific neutralizing antibodies. For example, the extent of antibody neutralization of HIV-1, SIV and SHIV can be measured in the TZM-bl cell line through the degree of luciferase reporter gene expression after infection. In the past, neutralization curves and titers for this standard assay have been calculated using an Excel macro. Updating all instances of such a macro with new techniques can be unwieldy and introduce non-uniformity across multi-lab teams. Using Excel also poses challenges in centrally storing, sharing and associating raw data files and results. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 6% |
Sweden | 1 | 3% |
Italy | 1 | 3% |
Unknown | 31 | 89% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 23% |
Researcher | 6 | 17% |
Student > Master | 4 | 11% |
Student > Postgraduate | 3 | 9% |
Student > Bachelor | 3 | 9% |
Other | 6 | 17% |
Unknown | 5 | 14% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 13 | 37% |
Medicine and Dentistry | 4 | 11% |
Biochemistry, Genetics and Molecular Biology | 3 | 9% |
Immunology and Microbiology | 3 | 9% |
Social Sciences | 2 | 6% |
Other | 4 | 11% |
Unknown | 6 | 17% |